Perform Bacterial LPS's: Immunohistochemistry in Ovaries
3:50
Detect 16S rRNA Sequencing
5:02
16S rRNA Sequencing Analysis
6:09
Predict Bacteria Function
6:36
Data
7:09
Representative Result
9:47
Conclusion
Transkript
This protocol is designed to discover bacteria in ovarian tissues and predict their functions. Immunohistochemistry staining and 16S rRNA sequencing, were used in order to discover and distinguish bacteria in cancerous and non-cancerous ovarian ti
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Immunohistochemistry staining and 16S ribosomal RNA gene (16S rRNA gene) sequencing were performed in order to discover and distinguish bacteria in cancerous and noncancerous ovarian tissues in situ. The compositional and functional differences of the bacteria were predicted by using BugBase and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt).