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Huadong Hospital Affiliated to Fudan University

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Isolation, Culture, and Adipogenic Induction of Neural Crest Original Adipose-Derived Stem Cells from Periaortic Adipose Tissue

Yiding Qi^*1, Xiang Miao^*2, Lian Xu³, Mengxia Fu³, Shi Peng⁴, Kailei Shi⁵, Jun Li⁴, Maoqing Ye⁵, Ruogu Li¹

¹Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, ²Shanghai Institute of Nutrition and Health, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, ³Shanghai Key Laboratory of Hypertension, Shanghai Institute of Hypertension, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, ⁴Department of Cardiology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, ⁵Department of Cardiology, Shanghai Key Laboratory of Clinical Geriatric Medicine, Huadong Hospital Affiliated to Fudan University

We present a protocol for the isolation, culture, and adipogenic induction of neural crest derived adipose-derived stem cells (NCADSCs) from the periaortic adipose tissue of Wnt-1 Cre^+/-;Rosa26^RFP/+ mice. The NCADSCs can be an easily accessible source of ADSCs for modeling adipogenesis or lipogenesis in vitro.

Education

Improved Renal Denervation Mitigated Hypertension Induced by Angiotensin II Infusion

Ming Wang^*1,2, Shuyi Zhang^*1,2, Wanling Han^1,2, Maoqing Ye^1,2, Xinkai Qu^1,2, Wenzheng Han^1,2

¹Department of Cardiology, Huadong Hospital Affiliated to Fudan University, ²Shanghai Key Laboratory of Clinical Geriatric Medicine

Here, we present a protocol for renal sympathetic denervation (RDN) in mice with hypertension induced by Angiotensin II infusion. The procedure is repeatable, convenient and allows to study the regulatory mechanisms of RDN on hypertension and cardiac hypertrophy.