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Bielefeld University

5 ARTICLES PUBLISHED IN JoVE

Neuroscience

Tactile Conditioning And Movement Analysis Of Antennal Sampling Strategies In Honey Bees (Apis mellifera L.)

Samir Mujagić¹, Simon Michael Würth¹, Sven Hellbach¹, Volker Dürr¹

¹Biological Cybernetics, CITEC - Cognitive Interaction Technology - Center of Excellence, Bielefeld University

In this protocol we show how to condition harnessed honey bees to tactile stimuli and introduce a 2D motion capture technique for analyzing the kinematics of fine-scale antennal sampling pattern.

Chemistry

Analysis of Volatile and Oxidation Sensitive Compounds Using a Cold Inlet System and Electron Impact Mass Spectrometry

Jens Sproß¹

¹Department of Chemistry, Institute of Organic Chemistry, Bielefeld University

This video presents a protocol for the mass spectrometrical analysis of volatile and oxidation sensitive compounds using electron impact ionization. The presented technique is especially of interest for inorganic chemists, working with metal organyls, silanes, or phosphanes which have to be handled using inert conditions, such as the Schlenk technique.

Behavior

Eye Tracking During Visually Situated Language Comprehension: Flexibility and Limitations in Uncovering Visual Context Effects

Camilo Rodriguez Ronderos¹, Katja Münster¹, Ernesto Guerra², Helene Kreysa³, Alba Rodríguez^1,4, Julia Kröger⁴, Thomas Kluth⁴, Michele Burigo⁴, Dato Abashidze⁵, Eva Nunnemann⁴, Pia Knoeferle^1,6,7

¹Institute of German Studies and Linguistics, Humboldt-Universität zu Berlin, ²Center for Advanced Research in Education – CIAE, Universidad de Chile, ³Institute of Psychology, Friedrich Schiller University Jena, ⁴Cognitive Interaction Technology Excellence Cluster (CITEC), Faculty of Linguistics and Literature, Bielefeld University, ⁵Department of Education, Concordia University, ⁶Berlin School of Mind and Brain, ⁷Einstein Center for Neurosciences Berlin

The present article reviews an eye-tracking methodology for studies on language comprehension. To obtain reliable data, key steps of the protocol must be followed. Among these are the correct set-up of the eye tracker (e.g., ensuring good quality of the eye and head images) and accurate calibration.

Biochemistry

Förster Resonance Energy Transfer Measurements in Living Plant Cells

Sonja Michèle Schmidtpott¹, Thorsten Seidel¹

¹Dynamic Cell Imaging, Biochemistry and Physiology of Plants, Faculty of Biology, Bielefeld University

A protocol is provided for setting up a standard confocal laser-scanning microscope for in vivo Förster resonance energy transfer measurements, followed by data evaluation.

Neuroscience

Neuronavigated Focalized Transcranial Direct Current Stimulation Administered During Functional Magnetic Resonance Imaging

Filip Niemann^*1, Alireza Shahbabaie^*1, Sven Paßmann¹, Steffen Riemann¹, Robert Malinowski¹, Harun Kocataş¹, Leonardo M. Caisachana Guevara^1,2, Mohamed Abdelmotaleb¹, Daria Antonenko¹, Felix Blankenburg^3,4, Rico Fischer², Gesa Hartwigsen^5,6, Shu-Chen Li^7,8, Michael A. Nitsche^9,10,11, Axel Thielscher^12,13, Dagmar Timmann¹⁴, Anna Fromm¹, Dayana Hayek¹, Ann-Kathrin Hubert¹, Andrew K. Martin^15,16, Alexander Hunold¹⁷, Agnes Flöel^*1,18, Marcus Meinzer^*1

¹Department of Neurology, University Medicine Greifswald, ²Department of Psychology, University Medicine Greifswald, ³Berlin School of Mind and Brain, Humboldt Universität zu Berlin, ⁴Neurocomputation and Neuroimaging Unit, Freie Universität Berlin, ⁵Wilhelm-Wundt-Institute for Psychology, Cognitive and Biological Psychology, Leipzig University, ⁶Lise Meitner Research Group Cognition and Plasticity, Max Planck Institute for Human Cognitive and Brain Sciences, ⁷Lifespan Developmental Neuroscience, Faculty of Psychology, Technische Universität Dresden, ⁸Centre for Tactile Internet with Human-in-the-Loop, Technische Universität Dresden, ⁹Department of Psychology and Neurosciences, Leibniz Research Centre for Working Environment and Human Factors, ¹⁰German Centre for Mental Health (DZPG), ¹¹Clinic of Psychiatry and Psychotherapy, Protestant Hospital of Bethel Foundation, University Hospital OWL, Bielefeld University, ¹²Danish Research Centre for Magnetic Resonance, Centre for Functional and Diagnostic Imaging and Research, Copenhagen University Hospital Amager and Hvidovre, ¹³Department of Health Technology, Technical University of Denmark, ¹⁴Department of Neurology and Center for Translational Neuro- and Behavioral Sciences (C-TNBS), Essen University Hospital, ¹⁵Department of Psychology, University of Kent, ¹⁶Kent Medway Medical School, ¹⁷Institute of Biomedical Engineering and Informatics, Technische Universität Ilmenau, ¹⁸German Center for Neurodegenerative Diseases (DZNE Site Greifswald)

This protocol describes the method of neuronavigated electrode placement for focal, transcranial direct current stimulation (tDCS) administered during functional magnetic resonance imaging (fMRI).