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The Johns Hopkins Hospital

5 ARTICLES PUBLISHED IN JoVE

Neuroscience

In vivo Electroporation of Developing Mouse Retina

Jimmy de Melo¹, Seth Blackshaw^1,2,3,4,5

¹Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine, ²Department of Neurology, Johns Hopkins School of Medicine, ³Department of Ophthalmology, Johns Hopkins School of Medicine, ⁴Center for High-Throughput Biology, Johns Hopkins School of Medicine, ⁵Institute for Cell Engineering, Johns Hopkins School of Medicine

A method for the incorporation of plasmid DNA into murine retinal cells for the purpose of performing either gain- or loss of function studies in vivo is presented. This method capitalizes on the transient increase in permeability of cell plasma membranes induced by the application of an external electrical field.

Neuroscience

Functional Interrogation of Adult Hypothalamic Neurogenesis with Focal Radiological Inhibition

Daniel A. Lee^1,2, Juan Salvatierra², Esteban Velarde³, John Wong³, Eric C. Ford⁴, Seth Blackshaw^2,5

¹Division of Biology, California Institute of Technology, ²Solomon H. Snyder Department of Neuroscience, Neurology, and Ophthalamology, Johns Hopkins University School of Medicine, ³Department of Radiation Oncology & Molecular Radiation Sciences, Johns Hopkins University School of Medicine, ⁴Department of Radiation Oncology, University Of Washington Medical Center, ⁵Institute for Cell Engineering and High-Throughput Biology Center, Johns Hopkins University School of Medicine

The function of adult-born mammalian neurons remains an active area of investigation. Ionizing radiation inhibits the birth of new neurons. Using computer tomography-guided focal irradiation (CFIR), three-dimensional anatomical targeting of specific neural progenitor populations can now be used to assess the functional role of adult neurogenesis.

Medicine

Dual-phase Cone-beam Computed Tomography to See, Reach, and Treat Hepatocellular Carcinoma during Drug-eluting Beads Transarterial Chemo-embolization

Vania Tacher¹, MingDe Lin², Nikhil Bhagat¹, Nadine Abi Jaoudeh³, Alessandro Radaelli⁴, Niels Noordhoek⁴, Bart Carelsen⁴, Bradford J. Wood³, Jean-François Geschwind¹

¹Russell H. Morgan Department of Radiology and Radiological Science, Division of Vascular and Interventional Radiology, The Johns Hopkins Hospital, ²Clinical Informatics, Interventional, and Translational Solutions (CIITS), Philips Research North America, ³Center for Interventional Oncology, Interventional Radiology Section, National Institutes of Health, ⁴Interventional X-ray, Philips Healthcare

Dual-phase cone-beam computed tomography (DP-CBCT) is a useful intraprocedural imaging technique for transarterial chemo-embolization treatment with drug-eluting beads of hepatocellular carcinoma. DP-CBCT has been used to perform three major steps in oncologic interventional radiology: tumor localization (see), navigation and intraprocedural catheter guidance (reach), and intraprocedural evaluation of treatment success (treat).

Medicine

Predicting Treatment Response to Image-Guided Therapies Using Machine Learning: An Example for Trans-Arterial Treatment of Hepatocellular Carcinoma

Aaron Abajian¹, Nikitha Murali¹, Lynn Jeanette Savic^1,2, Fabian Max Laage-Gaupp¹, Nariman Nezami¹, James S. Duncan³, Todd Schlachter¹, MingDe Lin⁴, Jean-François Geschwind⁵, Julius Chapiro¹

¹Department of Radiology and Biomedical Imaging, Yale School of Medicine, ²Department of Diagnostic and Interventional Radiology, Universitätsmedizin Charité Berlin, ³Department of Biomedical Engineering, Yale School of Engineering and Applied Science, ⁴Philips Research North America, ⁵Prescience Labs

Intra-arterial therapies are the standard of care for patients with hepatocellular carcinoma who cannot undergo surgical resection. A method for predicting response to these therapies is proposed. The technique uses pre-procedural clinical, demographic, and imaging information to train machine learning models capable of predicting response prior to treatment.

Neuroscience

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Kurt Weir^*1, Patrick Leavey^*1, Clayton Santiago¹, Seth Blackshaw^1,2,3,4,5,6

¹Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, ²Department of Psychiatry and Behavioral Science, The Johns Hopkins Hospital, ³Department of Ophthalmology, The Johns Hopkins Hospital, ⁴Department of Neurology, The Johns Hopkins Hospital, ⁵Institute for Cell Engineering, Johns Hopkins University School of Medicine, ⁶Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine

Here, the authors showcase the utility of MULTI-seq for phenotyping and subsequent paired scRNA-seq and scATAC-seq in characterizing the transcriptomic and chromatin accessibility profiles in retina.