University of Colorado, Boulder
3 ARTICLES PUBLISHED IN JoVE
Medicine
Assessment of Vascular Function in Patients With Chronic Kidney Disease
Kristen L. Jablonski 1, Emily Decker 1, Loni Perrenoud 1, Jessica Kendrick 1, Michel Chonchol 1, Douglas R. Seals 2, Diana Jalal 1
1Division of Renal Diseases and Hypertension, University of Colorado, Denver, 2Department of Integrative Physiology, University of Colorado, Boulder
The degree of vascular dysfunction and contributing physiological mechanisms can be assessed in patients with chronic kidney disease by measuring brachial artery flow-mediated dilation, aortic pulse-wave velocity, and vascular endothelial cell protein expression.
Biology
Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing
Jerome E. Lee 1, Rui Yi 1,2
1Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, 2Linda Crnic Institute for Down Syndrome, University of Colorado, Denver
MicroRNAs (miRNAs) are a widely conserved class of regulatory molecules. Here we describe a miRNA cloning method that relies upon two potent ligation steps followed by high-throughput sequencing. Our method permits accurate genome-wide quantitation of miRNAs.
Medicine
Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics
Russell T. Burke 1, James D. Orth 1
1Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder
Here, we describe a method of long-term time-lapse microscopy to longitudinally track single cells in response to anti-cancer therapeutics.
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