Isolation and Culture of Adult Zebrafish Brain-derived NeurospheresMiguel A. Lopez-Ramirez *1,2, Charles-Félix Calvo *3, Emma Ristori 1, Jean-Léon Thomas 1,3, Stefania Nicoli 1
1Yale Cardiovascular Research Center, Internal Medicine, Yale University, 2Department of Medicine, University of California, San Diego, 3APHP Groupe Hospitalier Pitié-Salpètrière, Université Pierre and Marie Curie
Here we provide a reproducible method to examine adult neurogenesis using a neurosphere assay derived from the whole brain or from either the telencephalic, tectal or cerebellar regions of the adult zebrafish brain. Additionally, we describe the procedure to manipulate gene expression in zebrafish neurospheres.
