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Guang'anmen Hospital of China Academy of Chinese Medical Science

3 ARTICLES PUBLISHED IN JoVE

Biology

In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability

Ban-Ruo Li^*1,2, Jia Wu^*1,2, Hua-Shan Li^1,2, Zhi-Hui Jiang^1,2, Xiu-Min Zhou^1,2, Cai-Hua Xu^1,2, Ning Ding^1,2, Juan-Min Zha^1,2, Wei-Qi He^1,2

¹Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda (CAM-SU) Genome Resource Center, Soochow University, ²Department of Oncology, The First Affiliated Hospital of Soochow University

Two methods are presented here to determine intestinal barrier function. An epithelial meter (volt/ohm) is used for measurements of transepithelial electrical resistance of cultured epithelia directly in tissue culture wells. In mice, the FITC-dextran gavage method is used to determine the intestinal permeability in vivo.

Behavior

Analysis of Learning and Memory Ability in an Alzheimer's Disease Mouse Model using the Morris Water Maze

Huiling Tian¹, Ning Ding², Mengwei Guo¹, Shun Wang¹, Zidong Wang¹, Hao Liu¹, Jiayi Yang¹, Yujie Li³, Jingyu Ren¹, Jing Jiang⁴, Zhigang Li¹

¹School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, ²Guang'anmen Hospital of China Academy of Chinese Medical Science, ³The Third Affiliated Hospital of Beijng University of Chinese Medicine, ⁴School of Nursing, Beijing University of Chinese Medicine

Herein, a protocol to conduct the Morris water maze tests to evaluate the ability of learning and memory of Alzheimer’s Disease model mice and to assess the effect of manual acupuncture for treating them is described.

Biology

Quantification of Proliferative and Dead Cells in Enteroids

Hua-Shan Li^*1, Shao-Fang Xu^*1, Jian-Ying Sheng^*1, Zhi-Hui Jiang¹, Jing Wang¹, Ning Ding¹, Tao Wang¹, Matthew A. Odenwald², Jerrold R. Turner^2,3, Wei-Qi He¹, Hong Xu¹, Juan-Min Zha¹

¹Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda (CAM-SU) Genomic Resource Center, Medical College of Soochow University, Department of Oncology, The First Affiliated Hospital of Soochow University, ²Department of Pathology, University of Chicago, ³Department of Pathology, Brigham and Women's Hospital–Harvard Medical School

The presented protocol uses flow cytometry to quantify the number of proliferating and dead cells in cultured mouse enteroids. This method is helpful to evaluate the effects of drug treatment on organoid proliferation and survival.