Flow Cytometry Analysis of EdU-Positive Cells in Enteroids
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Results: IL-22 Increases Enteroid Proliferation and Promotes Cell Death in Enteroids
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Conclusion
Transkript
This protocol is useful for enteroid isolation in a culture in addition to investigating cell proliferation and the survival in enteroids. Scientists can apply this technique to culture enteroids and to study the effect of drugs and inflammatory c
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The presented protocol uses flow cytometry to quantify the number of proliferating and dead cells in cultured mouse enteroids. This method is helpful to evaluate the effects of drug treatment on organoid proliferation and survival.