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Crown Bioscience Inc., Taicang, Jiangsu, China

2 ARTICLES PUBLISHED IN JoVE

Cancer Research

Immunophenotyping of Orthotopic Homograft (Syngeneic) of Murine Primary KPC Pancreatic Ductal Adenocarcinoma by Flow Cytometry

Xiaoyu An^*1,2, Xuesong Ouyang^*1, Hui Zhang¹, Tingting Li¹, Yu-yang Huang¹, Zhiyuan Li¹, Demin Zhou², Qi-Xang Li^1,2

¹Crown Bioscience Inc., ²State Key Laboratory of Natural and Biomimetic Drugs, Peking University

The experimental procedure on the immunophenotyping of murine orthotopic PDAC homografts aims at profiling the tumor immuno-microenvironment. Tumors are orthotopically implanted via surgery. Tumors of 200–600 mm³ in size were harvested and dissociated to prepare single-cell suspensions, followed by multi-immune marker FACS analysis using different fluorescently-labeled antibodies.

Cancer Research

Creating Matched In vivo/In vitro Patient-Derived Model Pairs of PDX and PDX-Derived Organoids for Cancer Pharmacology Research

Xiaoxi Xu¹, Limei Shang¹, Philip Wang², Jun Zhou², Xuesong Ouyang², Meiling Zheng¹, Binchen Mao², Likun Zhang², Bonnie Chen¹, Jingjing Wang², Jing Chen³, Wubin Qian², Sheng Guo², Yujun Huang², Qi-Xiang Li³

¹Crown Bioscience Inc., Changping District, Beijing, China, ²Crown Bioscience Inc., Taicang, Jiangsu, China, ³Crown Bioscience Inc., San Diego, CA, USA

A method is described to create organoids using patient-derived xenografts (PDX) for in vitro screening, resulting in matched pairs of in vivo/in vitro models. PDX tumors were harvested/processed into small pieces mechanically or enzymatically, followed by the Clevers’ method to grow tumor organoids that were passaged, cryopreserved and characterized against the original PDX.