Tumor Tissue Dissociation and Single Cell Preparation
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Flow Data Acquisition
6:46
Results: Comparison of Orthotopic and Subcutaneous Implantation of Pancreatic Ductal Adenocarcinoma
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Conclusion
Transkript
This message can help answer key questions in Immunophenotyping in Murine models, such as markers used to identify different immunity lineages and aging strategies. The major advantageous of this technique is that the marker and aging stategies, w
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The experimental procedure on the immunophenotyping of murine orthotopic PDAC homografts aims at profiling the tumor immuno-microenvironment. Tumors are orthotopically implanted via surgery. Tumors of 200–600 mm3 in size were harvested and dissociated to prepare single-cell suspensions, followed by multi-immune marker FACS analysis using different fluorescently-labeled antibodies.