Université Côte d’Azur, Inserm, C3M, Team Cellular and Molecular Pathophysiology of Obesity and Diabetes, Nice, France
2 ARTICLES PUBLISHED IN JoVE
Biology
Exploring Adipose Tissue Structure by Methylsalicylate Clearing and 3D Imaging
Jérôme Gilleron 1, Cindy Meziat 1, André Sulen 2, Stoyan Ivanov 3, Jennifer Jager 1, David Estève 4, Catherine Muller 4, Jean-Francois Tanti 1, Mireille Cormont 1
1Université Côte d'Azur, Inserm UMR1065, C3M, Team "Cellular and Molecular Pathophysiology of Obesity", Nice, France, 2Integrated Cardio Metabolic Center, Department of Medicine, Karolinska Institutet, Stockholm, Sweden, 3Université Côte d'Azur, Inserm UMR1065, C3M, Team "Haematometabolism in Diseases", Nice, France, 4Institut de Pharmacologie et de Biologie Structurale (IPBS), Université de Toulouse, CNRS, UPS, Toulouse, France
Here, we describe a simple, inexpensive and fast clearing method to resolve the 3D structure of both mouse and human white adipose tissue using a combination of markers to visualize vasculature, nuclei, immune cells, neurons, and lipid-droplet coat proteins by fluorescent imaging.
Biology
An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function
Jennifer Jager 1, Mélanie Gaudfrin 1, Jérôme Gilleron 1, Mireille Cormont 1, Jean-François Tanti 1
1Université Côte d’Azur, Inserm, C3M, Team Cellular and Molecular Pathophysiology of Obesity and Diabetes, Nice, France
Presented here is a protocol to deliver oligonucleotides such as small-interfering RNA (siRNA), micro-RNA mimics (miRs), or anti-micro-RNA (anti-miR) into mature adipocytes to modulate protein and micro-RNA expression.
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