University of Oklahoma, Norman
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
Chemical Cartography Approaches to Study Trypanosomatid Infection
Danya A. Dean *1,2, Jacob J. Haffner *2,3, Mitchelle Katemauswa 1, Laura-Isobel McCall 1,2,4
1Department of Chemistry and Biochemistry, University of Oklahoma, Norman, 2Laboratories of Molecular Anthropology and Microbiome Research, University of Oklahoma, Norman, 3Department of Anthropology, University of Oklahoma, Norman, 4Department of Microbiology and Plant Biology, University of Oklahoma, Norman
This protocol describes the steps to generate a 3D model of metabolite distribution during trypanosomatid infection, including sample collection, metabolite extraction, an overview of liquid chromatography-tandem mass spectrometry data acquisition, 3D model generation, and finally, data visualization.
Environment
Simulating Temperature in a Soil Incubation Experiment
Jianwei Li 1, Precious Areeveso 1, Xuehan Wang 1, Siyang Jian 1,2, Lahiru Gamage 1
1Department of Agricultural and Environmental Sciences, Tennessee State University, 2Department of Plant Biology and Microbiology, University of Oklahoma, Norman
Laboratory soil warming experiments usually employ two or more constant temperatures in multiple chambers. By presenting a sophisticated environmental chamber, we provide an accurate temperature control method to imitate the magnitude and amplitude of in situ soil temperature and improve the experimental design of soil incubation studies.
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