Accedi

University of Oklahoma, Norman

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Chemical Cartography Approaches to Study Trypanosomatid Infection

Danya A. Dean^*1,2, Jacob J. Haffner^*2,3, Mitchelle Katemauswa¹, Laura-Isobel McCall^1,2,4

¹Department of Chemistry and Biochemistry, University of Oklahoma, Norman, ²Laboratories of Molecular Anthropology and Microbiome Research, University of Oklahoma, Norman, ³Department of Anthropology, University of Oklahoma, Norman, ⁴Department of Microbiology and Plant Biology, University of Oklahoma, Norman

This protocol describes the steps to generate a 3D model of metabolite distribution during trypanosomatid infection, including sample collection, metabolite extraction, an overview of liquid chromatography-tandem mass spectrometry data acquisition, 3D model generation, and finally, data visualization.

Environment

Simulating Temperature in a Soil Incubation Experiment

Jianwei Li¹, Precious Areeveso¹, Xuehan Wang¹, Siyang Jian^1,2, Lahiru Gamage¹

¹Department of Agricultural and Environmental Sciences, Tennessee State University, ²Department of Plant Biology and Microbiology, University of Oklahoma, Norman

Laboratory soil warming experiments usually employ two or more constant temperatures in multiple chambers. By presenting a sophisticated environmental chamber, we provide an accurate temperature control method to imitate the magnitude and amplitude of in situ soil temperature and improve the experimental design of soil incubation studies.