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University Medical Center Rotterdam

2 ARTICLES PUBLISHED IN JoVE

Medicine

Surgical Placement of Catheters for Long-term Cardiovascular Exercise Testing in Swine

Daphne P M De Wijs-Meijler^*1, Kelly Stam^*2, Richard W B van Duin¹, Annemarie Verzijl², Irwin K Reiss³, Dirk J Duncker², Daphne Merkus²

¹Experimental Cardiology and Neonatology, Erasmus MC, ²Experimental Cardiology, Erasmus MC, ³Neonatology, Erasmus MC

Here we present a protocol to assess cardiopulmonary function in awake swine, at rest and during graded treadmill exercise. Chronic instrumentation allows for repeated hemodynamic measurements uninfluenced by cardiodepressive anesthetic agents.

Medicine

Preparation of Human Myocardial Tissue for Long-Term Cultivation

Jules Hamers^1,2, Payel Sen^1,2, Daphne Merkus^1,2,3, Thomas Seidel^4,5, Kun Lu^1,6, Andreas Dendorfer^1,2

¹Walter-Brendel-Centre of Experimental Medicine, University Hospital, LMU Munich, ²German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA), ³Division of Experimental Cardiology, Department of Cardiology, Erasmus MC, University Medical Center Rotterdam, ⁴Institute of Cellular and Molecular Physiology, Friedrich-Alexander-Universität Erlangen-Nürnberg, ⁵Muscle Research Center Erlangen (MURCE), Friedrich-Alexander-Universität Erlangen-Nürnberg, ⁶Department of Cardiac Surgery, University Hospital, LMU Munich

We present a protocol for ex vivo cultivation of human ventricular myocardial tissue. It allows for detailed analysis of contraction force and kinetics, as well as the application of pre- and afterload to mimic the in vivo physiological environment more closely.