University Medical Center Rotterdam
2 ARTICLES PUBLISHED IN JoVE
Medicine
Surgical Placement of Catheters for Long-term Cardiovascular Exercise Testing in Swine
Daphne P M De Wijs-Meijler *1, Kelly Stam *2, Richard W B van Duin 1, Annemarie Verzijl 2, Irwin K Reiss 3, Dirk J Duncker 2, Daphne Merkus 2
1Experimental Cardiology and Neonatology, Erasmus MC, 2Experimental Cardiology, Erasmus MC, 3Neonatology, Erasmus MC
Here we present a protocol to assess cardiopulmonary function in awake swine, at rest and during graded treadmill exercise. Chronic instrumentation allows for repeated hemodynamic measurements uninfluenced by cardiodepressive anesthetic agents.
Medicine
Preparation of Human Myocardial Tissue for Long-Term Cultivation
Jules Hamers 1,2, Payel Sen 1,2, Daphne Merkus 1,2,3, Thomas Seidel 4,5, Kun Lu 1,6, Andreas Dendorfer 1,2
1Walter-Brendel-Centre of Experimental Medicine, University Hospital, LMU Munich, 2German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA), 3Division of Experimental Cardiology, Department of Cardiology, Erasmus MC, University Medical Center Rotterdam, 4Institute of Cellular and Molecular Physiology, Friedrich-Alexander-Universität Erlangen-Nürnberg, 5Muscle Research Center Erlangen (MURCE), Friedrich-Alexander-Universität Erlangen-Nürnberg, 6Department of Cardiac Surgery, University Hospital, LMU Munich
We present a protocol for ex vivo cultivation of human ventricular myocardial tissue. It allows for detailed analysis of contraction force and kinetics, as well as the application of pre- and afterload to mimic the in vivo physiological environment more closely.
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