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The University of North Carolina at Greensboro

2 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Isolation of Preadipocytes from Broiler Chick Embryos
Minjeong Kim 1, Usuk Jung 1, Elizabeth Shepherd 1, Robert Mihelic 1, Brynn H. Voy 1
1Department of Animal Science, University of Tennessee

The present protocol describes a simple method for isolating preadipocytes from adipose tissue in broiler embryos. This method enables isolation with high yield, primary culture, and adipogenic differentiation of preadipocytes. Oil Red O staining and lipid/DNA stain measured the adipogenic ability of isolated cells induced with differentiation media.

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Neuroscience

Whole-Brain Single-Cell Imaging and Analysis of Intact Neonatal Mouse Brains Using MRI, Tissue Clearing, and Light-Sheet Microscopy
Felix A. Kyere *1,2, Ian Curtin *1,2, Oleh Krupa 1,2, Carolyn M. McCormick 1,2, Mustafa Dere 3, Sarah Khan 3,7, Minjeong Kim 7, Tzu-Wen Winnie Wang 4,5,6, Qiuhong He 4,5,6, Guorong Wu 3, Yen-Yu Ian Shih 4,5,6, Jason L. Stein 1,2
1UNC Neuroscience Center, University of North Carolina, Chapel Hill, 2Department of Genetics, University of North Carolina, Chapel Hill, 3Department of Psychiatry, University of North Carolina, Chapel Hill, 4Center for Animal Magnetic Resonance Imaging, The University of North Carolina at Chapel Hill, 5Biomedical Research Imaging Center, The University of North Carolina at Chapel Hill, 6Department of Neurology, The University of North Carolina at Chapel Hill, 7Department of Computer Science, The University of North Carolina at Greensboro

This protocol describes methods for conducting magnetic resonance imaging, clearing, and immunolabeling of intact mouse brains using iDISCO+, followed by a detailed description of imaging using light-sheet microscopy, and downstream analyses using NuMorph.

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