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The University of North Carolina at Greensboro

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Isolation of Preadipocytes from Broiler Chick Embryos

Minjeong Kim¹, Usuk Jung¹, Elizabeth Shepherd¹, Robert Mihelic¹, Brynn H. Voy¹

¹Department of Animal Science, University of Tennessee

The present protocol describes a simple method for isolating preadipocytes from adipose tissue in broiler embryos. This method enables isolation with high yield, primary culture, and adipogenic differentiation of preadipocytes. Oil Red O staining and lipid/DNA stain measured the adipogenic ability of isolated cells induced with differentiation media.

Neuroscience

Whole-Brain Single-Cell Imaging and Analysis of Intact Neonatal Mouse Brains Using MRI, Tissue Clearing, and Light-Sheet Microscopy

Felix A. Kyere^*1,2, Ian Curtin^*1,2, Oleh Krupa^1,2, Carolyn M. McCormick^1,2, Mustafa Dere³, Sarah Khan^3,7, Minjeong Kim⁷, Tzu-Wen Winnie Wang^4,5,6, Qiuhong He^4,5,6, Guorong Wu³, Yen-Yu Ian Shih^4,5,6, Jason L. Stein^1,2

¹UNC Neuroscience Center, University of North Carolina, Chapel Hill, ²Department of Genetics, University of North Carolina, Chapel Hill, ³Department of Psychiatry, University of North Carolina, Chapel Hill, ⁴Center for Animal Magnetic Resonance Imaging, The University of North Carolina at Chapel Hill, ⁵Biomedical Research Imaging Center, The University of North Carolina at Chapel Hill, ⁶Department of Neurology, The University of North Carolina at Chapel Hill, ⁷Department of Computer Science, The University of North Carolina at Greensboro

This protocol describes methods for conducting magnetic resonance imaging, clearing, and immunolabeling of intact mouse brains using iDISCO+, followed by a detailed description of imaging using light-sheet microscopy, and downstream analyses using NuMorph.