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Preparation of Bacterial Inoculum for Acinetobacter Biofilm Formation

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01:38 min

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August 4th, 2023

DOI :

10.3791/200399-v

August 4th, 2023

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Joo-Sung Kim¹^,², Jihoon Lim³

¹Korea Food Research Institute, ²Department of Food Biotechnology, Korea University of Science and Technology, ³Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute

Transkript

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Bacterial Inoculum Preparation For Acinetobacter Biofilm Formation 1 Inoculate A Blood Agar Plate With 2 10 L Of The Bacterial Strain Using A Sterile Pipette Tip Streak The Agar Surface

Thinning It Out With Intermittent Flaming Incubate The Plate At 30 C For 20 24 Hours 2 Select A Single Colony From The Agar Plate And Inoculate 5 ML Of Sterile BHI Broth Incubate The Inoculated Broth In A Shaking Incubator At 30 C

150 Rpm For 20 24 Hours 3 Transfer 1 ML Of The Culture To A Sterile Microcentrifuge Tube And Centrifuge At 6

000 G For 10 Minutes At Room Temperature Discard The Supernatant 4 Add 1 ML Of Sterile Phosphate buffered Saline PBS To The Pellet And Resuspend Using A Vortex Centrifuge At 6

000 G For 10 Minutes At Room Temperature And Discard The Supernatant 5 Resuspend The Resulting Pellet In Sterile

10 fold Diluted BHI Broth Using A Vortex Ensure The Optical Density Is Around 0 1 At 600 Nm

Seriden

Assessment of Acinetobacter Biofilm Formation and Characterization of Biofilms

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