The overall goal of this procedure is to generate chondrogenic lineage cells from human peripheral blood-derived induced pluripotent stem cells, or IPS cells. This method can help answer the key questions in the cartilage regeneration field about
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We present a protocol to generate a chondrogenic lineage from human peripheral blood (PB) via induced pluripotent stem cells (iPSCs) using an integration-free method, which includes embryoid body (EB) formation, fibroblastic cells expansion, and chondrogenic induction.