Homogenization and Fractionation of Rat Hippocampi
5:20
Isolation of the PSD from the Crude Membrane Protein Fraction
6:32
Results: Examination of Synaptic Proteins in Hippocampal S2, P2, and PSD Fractions from Rats that Received NS or Acute ECS
7:55
Conclusion
Transkript
The overall goal of the electroconvlusive seizure induction and subsequent smallscale fractionation of hippocampus is to induce global seizure activity in the rat brain, and to examine seizure activity induced alterations of proteins in the postsy
Bu içeriğe erişmek için giriş yapın veya ücretsiz denemenizi başlatın
Electroconvulsive seizure (ECS) is an experimental animal model of electroconvulsive therapy for severe depression. ECS globally stimulates activity in the hippocampus, leading to synaptogenesis and synaptic plasticity. Here, we describe methods for ECS induction in rats and for subcellular fractionation of their hippocampi to examine seizure-induced changes in synaptic proteins.