This video will illustrate how to capture tissue biopsies from the fovea, macula and peripheral retina for proteomic research. In several steps we'll use a four millimeter skin punch biopsy tool. The human eye is placed in a petri dish after it ha
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The human retina is composed of functionally and molecularly distinct regions, including the fovea, macula, and peripheral retina. Here, we describe a method using punch biopsies and manual removal of tissue layers from a human eye to dissect and collect these distinct retinal regions for downstream proteomic analysis.