Colorectal Cancer Cell (CRC) Organoid Artificial Extracellular Matrix Culture Installation
2:42
CRC Organic Cell-labeling with GFP Lentiviral Particles
5:28
GFP-labeled CRC Organoid Metastasis Generation
7:55
Results: Representative High-resolution GFP Visualization in Cultured GFP-labeled CRC Organoids, Primary Tumors, and Micrometastases
8:38
Conclusion
Transkript
This method can help answer key questions in the field of tumor biology about the micrometastases found in colon cancer patient. The main advantage of this technique is that it allows highly sensitive detection with micrometastases induced by GFP-
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To allow highly sensitive detection of the disseminating human colorectal cancer (CRC) cells colonizing tissues, we herein show a protocol for efficient transduction of green fluorescent protein (GFP) lentiviral particles into PDX-derived CRC organoid cells prior to their injection into recipient mice, with stereo-fluorescence microscopic observation.