More than one million uniform from the droplets are produced on the finger-sized substrate. And single DNA molecules are randomly distributed into each droplet. The protein yield is proportional to the number of the molecules in a droplet.
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The overall goal of the protocol is to prepare over one million ordered, uniform, stable, and biocompatible femtoliter droplets on a 1 cm2 planar substrate that can be used for cell-free protein synthesis.