The significance of this protocol is that it allows an accurate rheological characterization of non-Newtonian biological fluids, such as mucus, especially when only very small sample volumes are available. This technique facilitates the characteri
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This protocol presents a method to perform rheology characterization of mucus that resides on gill rakers (GRs) of the silver carp. Viscoelastic characteristics of GR-mucus, obtained by measuring viscosity, storage and loss moduli, are evaluated for the apparent yield stress to understand the filter feeding mechanism in GRs.