This method makes it possible to reproducibly quantify differences in gait parameters. The gait parameters can be assessed in freely-walking rodents, and the technique does not rely on invasive steps such as dipping the feet with ink. Demonstratin
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Here we describe the RatWalker system, built by redesigning the MouseWalker apparatus to accommodate for the increased size and weight of rats. This system uses frustrated total internal reflection (FTIR), high-speed video capture, and open-access analysis software to track and quantify gait parameters.