Stem Cell Passage and Differentiation and Definitive Endoderm Formation
3:46
Differentiation of Hepatic Progenitor Cells and Hepatocyte Differentiation
5:12
Results: Directed Hepatocyte-like Cell Induction from Human Embryonic Stem Cells
7:14
Conclusion
Transkript
Human embryonic stem cell-derived hepatocyte-like cells are useful in disease modeling and drug screening. This is an efficient and reproducible method for effectively inducing the differentiation of human embryonic stem cells into functional hepa
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This manuscript describes a detailed protocol for differentiation of human embryonic stem cells (hESCs) into functional hepatocyte-like cells (HLCs) by continuously supplementing Activin A and CHIR99021 during hESC differentiation into definitive endoderm (DE).