Canlı Çekim Melanom Hastalarından Beyin Omurilik Sıvısında Dolaşan Tümör Hücrelerinin Melanomla İlişkili Leptomeningeal Hastalığı İncelemek İçin Kültürü

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06:25 min

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March 29th, 2024

DOI :

10.3791/66071-v

March 29th, 2024

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Vincent Law¹^,², Inna Smalley³, Brittany R. Evernden², Margaret Baldwin⁴, Keiran S. M. Smalley¹, Peter A. Forsyth¹^,²

¹Department of Tumor Biology, University of South Florida, H. Lee Moffitt Cancer Center & Research Institute, ²Department of Neuro-Oncology, University of South Florida, H. Lee Moffitt Cancer Center & Research Institute, ³Department of Cancer Physiology, University of South Florida, H. Lee Moffitt Cancer Center & Research Institute, ⁴Department of Comparative Medicine, University of South Florida, H. Lee Moffitt Cancer Center & Research Institute

Transkript

Özet

Daha Fazla Video Keşfet

Anahtar Kelimeler Dola mdaki T m r H creleri

Serebral Omurilik S v s

Melanom

Leptomeningeal Hastal k

Ex Vivo K lt r

nsan Meningeal H creleri

ns lin Benzeri B y me Fakt r

MAPK Sinyali

Fare Modeli

Bu videodaki bölümler

0:00

Introduction

1:36

Preparation of Human Meningeal Cell (HMC) Conditioned Media and Collection of Cerebrospinal Fluid for the Culture of Circulating Tumor Cells

3:23

In Vitro Culture and Expansion of Cerebro Spinal Fluid (CSF)-Circulating Tumor Cells (CTCs) for the Study of Leptomeningeal Disease