Electromagnetic (EM) radiation can be considered an oscillating electric and magnetic field propagating through a medium that can interact with matter in its path. The electric field in the radiation can interact with electrical charges in the atoms or molecules in the matter. On the other hand, the magnetic field can interact with the magnetic field in the atomic nucleus. The study of the interaction between electromagnetic radiation and matter is termed spectroscopy. Spectroscopy is the study of how EM radiation interacts with matter, primarily focusing on how substances absorb and emit light. This process reveals unique information about the atomic and molecular structure of the matter involved, providing insight into its chemical composition and properties.

EM radiation can be considered as a wave and a particle simultaneously. It can be characterized by the properties of a wave, such as intensity (I) and amplitude (A). However, its interactions with matter are often thought of in terms of photons, which are elementary particles. Intensity (or irradiance) is the number of photons striking a given matter area during a particular time interval. The amplitude of the wave is the distance between the peak of the wave and the equilibrium point of the wave. The intensity of radiation is directly proportional to the square of its amplitude.

EM radiation may be transmitted through matter without any interaction. In this case, the intensity and energy of the radiation going into the matter and coming out of the matter will be the same. The interaction between EM radiation and matter can occur in different ways. In some cases, transmission occurs, allowing radiation to pass through matter without alteration, maintaining the same intensity and energy levels upon exit. Reflection and scattering are other interaction types where radiation is directed back to its source or dispersed randomly; the energy may remain unchanged (elastic) or may vary (inelastic). Another significant interaction is absorption, where matter takes up energy from the radiation, resulting in a reduction of the transmitted radiation's intensity. Emission occurs when absorbed energy is later released by the substance, often as light at different wavelengths.

To analyze such interactions, various spectroscopic techniques are used in research and industry, including infrared (IR) spectroscopy, ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, and mass spectrometry (MS). Each technique offers a unique window into molecular structure, enabling precise identification of compounds and detailed analysis of complex chemical systems.