February 22nd, 2010
•Many plant tissues, including phloem and xylem from loblolly pine (Pinus taeda L.), contain high levels of phenolics and polysaccharides that interfere with RNA purification. This presentation discusses techniques for the harvest of field-grown tissues and isolation of RNA of sufficient quality for microarrays and other genomic analyses.
Tags
Related Videos
Processing the Loblolly Pine PtGen2 cDNA Microarray
RNA Isolation from Embryonic Zebrafish and cDNA Synthesis for Gene Expression Analysis
Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry (CCMS)
A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals
Isolation and Purification of Kinesin from Drosophila Embryos
Vampiric Isolation of Extracellular Fluid from Caenorhabditis elegans
An Improved Method for Accurate and Rapid Measurement of Flight Performance in Drosophila
RNA Isolation from Mouse Pancreas: A Ribonuclease-rich Tissue
Hybrid-Cut: An Improved Sectioning Method for Recalcitrant Plant Tissue Samples
Stomata Tape-Peel: An Improved Method for Guard Cell Sample Preparation
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved