JoVE Logo
Faculty Resource Center

Sign In

A Stainless Protocol for High Quality RNA Isolation from Laser Capture Microdissected Purkinje Cells in the Human Post-Mortem Cerebellum

DOI :

10.3791/58953-v

January 17th, 2019

January 17th, 2019

7,194 Views

1Department of Pathology and Cell Biology, Columbia University, 2Division of Movement Disorders, Department of Neurology, Yale University, 3Department of Chronic Disease Epidemiology, Yale School of Public Health, Yale University, 4Center for Neuroepidemiology and Clinical Neurological Research, Yale School of Medicine, Yale University

This protocol uses a stain-free approach to visualize and isolate Purkinje cells in fresh-frozen tissue from human post-mortem cerebellum via laser capture microdissection. The purpose of this protocol is to generate sufficient amounts of high-quality RNA for RNA-sequencing.

Tags

Stainless Protocol

-- Views

Related Videos

article

Laser Capture Microdissection of Drosophila Peripheral Neurons

article

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling

article

High-resolution Functional Magnetic Resonance Imaging Methods for Human Midbrain

article

Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube

article

Laser Capture Microdissection of Neurons from Differentiated Human Neuroprogenitor Cells in Culture

article

Production of RNA for Transcriptomic Analysis from Mouse Spinal Cord Motor Neuron Cell Bodies by Laser Capture Microdissection

article

Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells

article

Isolation of Distinct Cell Populations from the Developing Cerebellum by Microdissection

article

Laser Capture Microdissection - A Demonstration of the Isolation of Individual Dopamine Neurons and the Entire Ventral Tegmental Area

article

A Simple One-step Dissection Protocol for Whole-mount Preparation of Adult Drosophila Brains

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved