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48.2K Views
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10:00 min
April 28th, 2007
DOI :
10.3791/184-v
Chapters
0:06
Introduction
1:09
Tools and Reagents
2:17
Dissection procedure
4:43
Removal of epididymis
7:58
Visualization of sperm
8:20
Insemination of females
9:27
Conclusion
Summary
This protocol demonstrates methods for extracting sperm from the testes of males and then inseminating female mice. This procedure is useful when precise time is needed in developmental studies as well as transgenic work.
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