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Plastic Embedding and Sectioning of Xenopus laevis Embryos

DOI :

10.3791/188-v

April 29th, 2007

April 29th, 2007

13,387 Views

1Department of Developmental and Cell Biology, University of California, Irvine (UCI), 2University of California, Irvine (UCI)

Plastic sections maintain true tissue morphology in thin sections of tissue that can be immunostained with fluorescent secondary antibodies, making this method more useful than paraffin-embedded or frozen sections for many types of tissue. The method for staining, plastic embedding, and sectioning is demonstrated in this video.

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Plastic Embedding

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