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Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells in Xenopus laevis

DOI :

10.3791/1949-v

May 23rd, 2010

May 23rd, 2010

14,907 Views

1Bioengineering, University of Pittsburgh, 2Developmental Biology, University of Pittsburgh

Xenopus embryonic epithelia are an ideal model system to study cell behaviors such as polarity development and shape change during epithelial morphogenesis. Traditional histology of fixed samples is increasingly being complemented by live-cell confocal imaging. Here we demonstrate methods to isolate frog tissues and visualize live epithelial cells and their cytoskeleton using live-cell confocal microscopy.

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Live cell Imaging

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