This work was supported by National Institutes of Health R01 NS104829 (B.E.P.), R01 MH117149 (L.J.V.), and F99NS12053 (L.D.Q.) and the UT Southwestern GSO Endowment Award (R.J.P. and L.D.Q.). The authors thank Jenny Scaria (Texas Tech University Health Sciences Center School of Pharmacy) for technical assistance and Dr. Brendon Watson (University of Michigan) for methodological suggestions.

The present study was approved by the University of Texas Southwestern Medical Center Institutional Animal Care and Use Committee (protocol 2015-100867) and performed in compliance with both institutional and National Institute of Health guidelines. The C57/Bl6 male and female mice used in the present study were implanted at p20 (weight 8.3-11.1 g at the time of implantation).
1. Micro-drive design and construction
<ol>
	<li>Digitally designing and printing the micro-drive (Figure 1)
	<ol>
		<li>Download the micro-drive model templates (https://github.com/Brad-E-Pfeiffer/JuvenileMouseMicroDrive).</li>
		<li>Identify the stereotaxic locations of the target brain region(s) in an appropriate stereotaxic atlas.</li>
		<li>Using three-dimensional computer-aided design (3D CAD) software, load the template micro-drive cannula (Figure 1B).</li>
		<li>If necessary, modify the output cannula output locations on the micro-drive cannula model to target the desired brain region(s). 
		NOTE: Each cannula hole extrusion should be at least 2 mm long to ensure that the tetrode will emerge from the cannula hole aiming straight at the target. The micro-drive cannula template is designed to bilaterally target the anterior cingulate cortex (one tetrode per hemisphere), hippocampal area CA1 (four tetrodes per hemisphere), and hippocampal area CA3 (two tetrodes per hemisphere), with one reference tetrode per hemisphere positioned in the white matter above hippocampal area CA1.</li>
		<li>If necessary, modify the micro-drive body (Figure 1A) to accommodate the attachment of the electronic interface board (EIB).</li>
		<li>Print the micro-drive body, cannula, cone, and lid at high resolution on a 3D printer (ideally with a resolution better than 25 &#181;m), and prepare the printed materials according to the manufacturer&#39;s protocols. Use printer resins with high rigidity.</li>
	</ol>
	</li>
	<li>Assembling the custom screws and attachments (Figure 2A, B)
	<ol>
		<li>Using 3D CAD software, load the screw attachment models (Figure 1E).</li>
		<li>Print the screw attachments at high resolution on a 3D printer (ideally with a resolution of at least 25 &#181;m), and prepare the printed materials according to the manufacturer&#39;s protocols. Use printer resins with high rigidity.</li>
		<li>Affix the screw attachments to each tetrode-advancing screw (Figure 1F) (tetrode-advancing screws are custom manufactured at a machine shop prior to micro-drive construction).
		<ol>
			<li>Affix two screw attachments to each screw, with one above and one below the ridge. Ensure that the bottom of each screw attachment contacts the ridge. Hold the screw attachments together with gel cyanoacrylate.</li>
			<li>Once affixed, ensure that the screw attachments do not move in the longitudinal axis of the screw but rotate freely with minimal resistance.</li>
		</ol>
		</li>
	</ol>
	</li>
	<li>Assembling the micro-drive body (Figure 2C, D)
	<ol>
		<li>Using fine, sharp scissors, cut large polyimide tubing (outer diameter: 0.2921 mm, inner diameter: 0.1803 mm) into ~6 cm long sections.</li>
		<li>Pass the large polyimide sections through the output holes on the micro-drive cannula so that each tube extends beyond the bottom of the cannula by a few millimeters.</li>
		<li>Using a clean 30 G needle, affix the polyimide to the cannula by applying small amounts of liquid cyanoacrylate. Take care not to allow the cyanoacrylate to enter the inside of the polyimide tube. 
		NOTE: Dripping liquid cyanoacrylate down into the cannula through the top of the drive body can expedite this process but will require re-clearing of the guide holes later with a fine-tipped drill.</li>
		<li>Pass the large polyimide tubes from the top of the micro-drive cannula through the appropriate large polyimide holes in the micro-drive body.</li>
		<li>Slowly push the micro-drive cannula and micro-drive body together until they are adjacent and the cannula/body attachment tabs interlock. Be careful not to kink or damage the polyimide tubes in the process. 
		NOTE: Each polyimide tube should smoothly pass from the bottom of the cannula out through the top of the micro-drive body. Slight bending is normal, but excessive bending of the polyimide tube can warp the tetrode and prevent it from passing straight into the brain.</li>
		<li>Affix the micro-drive body and the micro-drive cannula together using cyanoacrylate.</li>
		<li>Using a new, sharp razor blade, sever the large polyimide tube ends that are extruding from the bottom of the cannula output holes. Ensure that the cut is exactly at the base of the cannula, making the tubes and the cannula bottom flush with one another.</li>
		<li>Using sharp scissors, cut the large polyimide tubing just above the edge of the inner rim of the drive body at a ~45&#176; angle.</li>
	</ol>
	</li>
	<li>Loading the assembled custom screws (Figure 2E)
	<ol>
		<li>Screw each assembled custom screw into the outer holes of the micro-drive body. Ensure the screw guidepost passes through the large hole in the screw attachments. Advance each screw fully until it will not advance further. Pre-lubricating the screws with mineral oil or axle grease is recommended.</li>
		<li>Using extremely sharp scissors, cut small polyimide tubing (outer diameter: 0.1397 mm, inner diameter: 0.1016) into ~4 cm long sections.</li>
		<li>Pass the small polyimide sections through the large polyimide tubing already mounted in the micro-drive. Ensure that excess small polyimide tubing protrudes from the top and bottom of each large polyimide tube.</li>
		<li>Affix the small polyimide tubes to the screw attachments via cyanoacrylate, taking care not to let any cyanoacrylate enter into either the large or small polyimide tubes.</li>
		<li>Using a new, sharp razor blade, sever the small polyimide tube ends that are extruding from the bottom of the cannula holes. Ensure that the cut is exactly at the base of the cannula and that the cut is clean, with nothing blocking the polyimide tube hole.</li>
		<li>Using sharp scissors, cut the top of the small polyimide a few millimeters above the top of the screw attachment at a ~45&#176; angle. Ensure the cut is clean, with nothing blocking the polyimide tube hole.</li>
	</ol>
	</li>
	<li>Loading the tetrodes
	<ol>
		<li>Prepare the tetrodes (~6 cm in length) using previously described methods31.</li>
		<li>Using ceramic- or rubber-tipped forceps, carefully pass a tetrode through one of the small polyimide tubes, leaving ~2 cm protruding from the top of the small polyimide tube.</li>
		<li>Affix the tetrode to the top of the small polyimide tube via liquid cyanoacrylate, taking care not to affix the small and large polyimide tubes together in the process.</li>
		<li>Retract the screw until it is near the top of the drive.</li>
		<li>Grab the tetrode wire protruding from the bottom of the drive, and gently kink it at the point where it emerges from the cannula.</li>
		<li>Advance the screw fully back into the drive.</li>
		<li>Using very sharp scissors, cut the tetrode wire just above the kink. Under a microscope, ensure that the cut is clean and that the metal of all four tetrodes is exposed.</li>
		<li>Retract the screw until the tetrode is just secured within the cannula.</li>
		<li>Repeat steps 1.5.2-1.5.8 for all the screws.</li>
		<li>Attach the EIB to the EIB support platform via small jewelry screws.</li>
		<li>Connect each electrode of each tetrode to the appropriate port on the EIB.</li>
	</ol>
	</li>
	<li>Preparing the micro-drive for surgery
	<ol>
		<li>Electrically plate the tetrodes to reduce the electrical impedance using previously described methods31.</li>
		<li>After plating, ensure that each tetrode is housed in the cannula such that the tip of the tetrode is flush with the bottom of each cannula hole.</li>
		<li>Slide the micro-drive cone around the completed micro-drive. Attach the micro-drive lid to the micro-drive cone by sliding the cone attachment pole into the lid port.</li>
		<li>Orient the cone so that the EIB connectors pass freely through the EIB connection pass-through holes when the lid is closed, and glue the cone in place with cyanoacrylate placed around the base of the cone, taking care not to let any cyanoacrylate enter into any of the cannula output holes. Remove the lid.</li>
		<li>Carefully backfill each cannula hole with sterile mineral oil to prevent bodily fluids from entering the polyimide holes after surgical implantation.</li>
		<li>Carefully coat the base of the cannula with sterile petroleum jelly. This will serve as a barrier to prevent chemical agents (e.g., dental cement) from entering the exposed brain during the surgery.</li>
		<li>Weigh the fully assembled micro-drive, lid, and four bone screws to prepare an equal-weight counterbalance.</li>
		<li>Optionally, prior to the surgery, extrude the tetrodes at a distance that is appropriate for reaching the target brain regions once the drive is flush with the skull. 
		NOTE:&#160;Prior to surgical implantation, sterilize the implant via gas sterilization in ethylene oxide (500-1200 mg/L, 2-4 hrs).&#160; All bone screws and surgical instruments should be sterilized via autoclave (121&#176;C, 30 minutes).</li>
	</ol>
	</li>
</ol>
2. Surgical implantation
<ol>
	<li>Anesthetizing the mouse and mounting it in the stereotaxic apparatus
	<ol>
		<li>Place the mouse in a small box with sufficient space to move, and anesthetize the mouse with 3%-4% isoflurane. 
		NOTE: Other anesthetic agents can be used, but caution should be used due to the age, size, and weight of the juvenile mouse subject.</li>
		<li>Once the mouse is unresponsive (no response to tail pinch, a ventilation rate of ~60 breaths per minute), remove it from the box, and quickly mount it on the stereotaxic apparatus.</li>
		<li>Quickly, place the stereotaxic mask over the mouse&#8217;s snout and maintain anesthesia at&#160;1-3% isoflurane. Apply any veterinary-approved pain relief, such as sustained-release buprenorphine (0.05-0.5 mg/kg subcutaneous), or anti-inflammatory agents, such as carprofen (5-10 mg/kg subcutaneous), prior to initial surgical incision.</li>
		<li>Fully secure the mouse&#39;s head in the stereotaxic apparatus using ear bars. Ensure the skull is level and immobile without placing unnecessary pressure on the mouse&#39;s ear canals. Due to the limited ossification of juvenile skull bones, it is possible to cause permanent damage during head fixation.</li>
	</ol>
	</li>
	<li>Preparing the mouse for surgery and exposing the skull
	<ol>
		<li>Protect the mouse&#39;s eyes by placing a small volume of synthetic tear gel on each eye and covering each eye with an autoclaved patch of foil. 
		&#8203;NOTE: The synthetic tears will keep the eyes moist, while the foil will prevent any light source from causing long-term damage. Thicker synthetic tear solutions are preferred as they can also serve as a barrier to the inadvertent introduction of other potentially toxic surgical solutions (ethanol, dental acrylic, etc.) into the eyes.</li>
		<li>Using sterile cotton-tipped swabs, apply hair-removal cream over the surgical area to remove the hair from the scalp. Be&#160;careful not to get the cream near the eyes.&#160;After removing the hair, place a&#160;sterile drape over the scalp to secure the surgical area.</li>
		<li>Using sterile cotton-tipped swabs, clean the scalp via three consecutive washes of povidone-iodine (10%) solution followed by isopropyl alcohol (100%).</li>
		<li>Using a sterile scalpel or fine scissors, remove the scalp.</li>
		<li>Using sterile cotton-tipped swabs and sterile solutions of saline solution (0.9% NaCl) and hydrogen peroxide, thoroughly clean the skull.</li>
		<li>Identify bregma, and using the stereotaxic apparatus, carefully mark the target recording locations on the skull with a permanent marker.</li>
	</ol>
	</li>
	<li>Opening the cannula hole and attaching the bone anchors
	<ol>
		<li>Remove the skull overlaying the recording sites. # Due to the thinness of the skull at this age, cut the skull with a scalpel blade; this removes the necessity of using a drill, which may damage the underlying dura. Keep the exposed dura moist with the application of sterile saline solution (0.9% NaCl) or sterile mineral oil. Do not remove or puncture the dura at this stage, as it is sufficiently thin in juvenile mice for the tetrodes to pass through in future steps.</li>
		<li>Carefully drill pilot holes for four bone screws.
		<ol>
			<li>Place the bone screws in the extreme lateral and rostral or caudal portions of the skull, where the bone is thickest and the bone screws are sufficiently far away from the micro-drive implant. For the bone screws, use sterile, fine jewelry screws (e.g.,&#160;UNM 120 thread, 1.5 mm head).</li>
			<li>Tightly wind one bone screw with a thin, highly conductive wire that will serve as a ground and be attached to the EIB in step 2.4.6.</li>
		</ol>
		</li>
		<li>Using a scalpel blade or carefully using a drill bit, score the skull near the bone screw hole locations. Scoring is important to provide a sufficiently rough surface for the liquid cyanoacrylate to bind in step 2.3.5.</li>
		<li>Using a sterile screwdriver and sterile screw clamp, thread each sterile bone screw into place, taking care not to pierce the underlying dura.</li>
		<li>Using a sterile 30 G needle, place liquid cyanoacrylate around each bone screw. This effectively thickens the skull where the bone screws have been attached. Take care not to allow any cyanoacrylate to enter into the exposed dura above the recording sites.</li>
	</ol>
	</li>
	<li>Lowering and attaching the micro-drive (Figure 2G)
	<ol>
		<li>Mount the completed micro-drive onto the stereotaxic apparatus to be carefully lowered onto the mouse skull. Ensure that the micro-drive cannula will be at the appropriate coordinates when lowered.</li>
		<li>Lower the micro-drive slowly, moving only in the dorsal/ventral direction. Lower the micro-drive with the tetrodes already advanced out of the cannula holes (step 1.6.6) in order to visualize their entry into the brain; any medial/lateral or rostral/caudal movement when the tetrodes are touching the mouse can bend the tetrodes and cause them to miss their final destination.</li>
		<li>Once the micro-drive is fully lowered, ensure that the base of the cannula just makes contact with the skull/dura. The layer of petroleum jelly and/or mineral oil will serve as a barrier to cover the exposed dura. If necessary, add sterile petroleum jelly or sterile bone wax to cover excess exposed dura.</li>
		<li>While holding the micro-drive in place with the stereotaxic apparatus, coat the skull in dental cement to affix the micro-drive base to the implanted bone screws. 
		NOTE: The dental cement should fully encase all the bone screws and should cover the dental cement anchor ledge on the micro-drive cannula.</li>
		<li>While the dental cement sets, carefully shape it to prevent sharp corners or edges that may harm the mouse or damage the micro-drive. Ensure there is sufficient dental cement to hold the micro-drive, but eliminate excessive dental cement that will add unnecessary weight.</li>
		<li>Carefully thread the ground wire through the micro-drive, and attach it to the appropriate slot on the EIB.</li>
		<li>Once the dental cement is fully set, carefully detach the micro-drive from the stereotaxic apparatus. Place the lid on the micro-drive.</li>
		<li>With a sterile cotton-tipped swab and sterile saline, clean the mouse.</li>
		<li>With a sterile cotton-tipped swab, apply a thin layer of antibiotic ointment to any exposed scalp near the implant site.</li>
		<li>Remove the foil from the mouse&#39;s eyes.</li>
		<li>Remove the mouse from the stereotaxic apparatus, taking care to support the additional weight of the micro-drive as the mouse is transported to a clean cage.</li>
	</ol>
	</li>
</ol>
3. Post-surgery recovery
<ol>
	<li>Immediate recovery
	<ol>
		<li>Prior to the surgery, prepare the counterbalance system by connecting a 0.75 in diameter PVC pipe, as shown in Figure 2G. One arm of the system passes through holes drilled into the lid of the cage, the second arm rests on top of the cage lid, and the third arm extends above and beyond the cage. The topmost arm is capped.</li>
		<li>Carefully attach the micro-drive to the counterbalance system (Figure 2G-I), and use a counterbalance weight identical to the weight of the micro-drive and bone screws. Run a strong thread or fishing line from a connector attached to the EIB over the three arms of the counterbalance system to the counterbalance weight, which hangs over the topmost arm.</li>
		<li>Ensure that the counterbalance is strongly connected to the micro-drive EIB and that there is sufficient line to give the mouse complete access to the entirety of the cage.</li>
		<li>Provide nutrient-rich gel in the cage alongside moistened normal rodent chow to ensure rehydration and recovery.</li>
		<li>Monitor the mouse until it fully recovers from the surgical anesthesia.</li>
	</ol>
	</li>
	<li>Long-term recovery
	<ol>
		<li>At all times, when not attached to the recording equipment, ensure that the micro-drive is supported by the counterbalance system. Reduce the counterweight over time, but never remove it entirely to avoid unanticipated stress to the mouse or torque to the bone screws.</li>
		<li>To prevent damage to the implant and counterbalance system, house the mouse without the possibility of direct interaction with other mice for the duration of the experiment.</li>
		<li>Provide nutrient-rich gel for at least 3 days following surgery, at which point solid food alone will be sufficient.
		<ol>
			<li>Due to the overhead requirements of the counterbalance system, do not provide food and water in an overhead wire mesh; place the food on the cage floor, and provide water through the side of the cage. To prevent spoilage, replace the food entirely daily.</li>
		</ol>
		</li>
		<li>Daily, ensure that the mouse has free access to the entirety of the cage and that the counterbalance is robustly and strongly attached to the micro-drive.</li>
	</ol>
	</li>
</ol>

Micro-Drive Implant for Recording Daily Neural Activity in Juvenile Mice

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(26), e1094 (2009).</li><li>Buzs&#225;ki, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Large-scale+recording+of+neuronal+ensembles.">Large-scale recording of neuronal ensembles.</a> Nature Neuroscience. 7 (5), 446-451 (2004).</li><li>Farooq, U., Dragoi, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Emergence+of+preconfigured+and+plastic+time-compressed+sequences+in+early+postnatal+development.">Emergence of preconfigured and plastic time-compressed sequences in early postnatal development.</a> Science. 363 (6423), 168-173 (2019).</li><li>Langston, R. F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+the+spatial+representation+system+in+the+rat.">Development of the spatial representation system in the rat.</a> Science. 328 (5985), 1576-1580 (2010).</li><li>Wills, T. J., Cacucci, F., Burgess, N., O'Keefe, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+the+hippocampal+cognitive+map+in+preweanling+rats.">Development of the hippocampal cognitive map in preweanling rats.</a> Science. 328 (5985), 1573-1576 (2010).</li><li>Bjerknes, T. L., Moser, E. I., Moser, M. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Representation+of+geometric+borders+in+the+developing+rat.">Representation of geometric borders in the developing rat.</a> Neuron. 82 (1), 71-78 (2014).</li><li>Bjerknes, T. L., Dagslott, N. C., Moser, E. I., Moser, M. -. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Path+integration+in+place+cells+of+developing+rats.">Path integration in place cells of developing rats.</a> Proceedings of the National Academy of Sciences. 115 (7), E1637-E1646 (2018).</li><li>Jansen, N. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Impaired+&#952;-&#947;+coupling+indicates+inhibitory+dysfunction+and+seizure+risk+in+a+Dravet+syndrome+mouse+model.">Impaired &#952;-&#947; coupling indicates inhibitory dysfunction and seizure risk in a Dravet syndrome mouse model.</a> Journal of Neuroscience. 41 (3), 524-537 (2021).</li><li>Nguyen, D. P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Micro-drive+array+for+chronic+in+vivo+recording:+Tetrode+assembly.">Micro-drive array for chronic in vivo recording: Tetrode assembly.</a> Journal of Visualized Experiments. (26), e1098 (2009).</li><li>Voigts, J., Siegle, J., Pritchett, D. L., Moore, C. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+flexDrive:+An+ultra-light+implant+for+optical+control+and+highly+parallel+chronic+recording+of+neuronal+ensembles+in+freely+moving+mice.">The flexDrive: An ultra-light implant for optical control and highly parallel chronic recording of neuronal ensembles in freely moving mice.</a> Frontiers in Systems Neuroscience. 7, 8 (2013).</li><li>Voigts, J., Newman, J. P., Wilson, M. A., Harnett, M. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+easy-to-assemble,+robust,+and+lightweight+drive+implant+for+chronic+tetrode+recordings+in+freely+moving+animals.">An easy-to-assemble, robust, and lightweight drive implant for chronic tetrode recordings in freely moving animals.</a> Journal of Neural Engineering. 17 (2), 026044 (2020).</li><li>Guardamagna, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Hybrid+Drive:+A+chronic+implant+device+combining+tetrode+arrays+with+silicon+probes+for+layer-resolved+ensemble+electrophysiology+in+freely+moving+mice.">The Hybrid Drive: A chronic implant device combining tetrode arrays with silicon probes for layer-resolved ensemble electrophysiology in freely moving mice.</a> Journal of Neural Engineering. 19 (3), (2022).</li><li>Yamamoto, J., Wilson, M. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Large-scale+chronically+implantable+precision+motorized+microdrive+array+for+freely+behaving+animals.">Large-scale chronically implantable precision motorized microdrive array for freely behaving animals.</a> Journal of Neurophysiology. 100 (4), 2430-2440 (2008).</li><li>Vora, S. R., Camci, E. D., Cox, T. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Postnatal+ontogeny+of+the+cranial+base+and+craniofacial+skeleton+in+male+C57BL/6J+mice:+A+reference+standard+for+quantitative+analysis.">Postnatal ontogeny of the cranial base and craniofacial skeleton in male C57BL/6J mice: A reference standard for quantitative analysis.</a> Frontiers in Physiology. 6, 417 (2016).</li></ol>

The authors have nothing to disclose.

Modern experiments exploring in vivo neural circuit function in rodents often utilize extracellular electrophysiology via permanently implanted electrodes to monitor the activity of individual neurons (i.e., single units) or&#160;local populations (via local field potentials, LFP), but such methods are rarely applied to juvenile mice due to technical challenges. This manuscript describes a method for obtaining in vivo electrophysiological recordings in mice across the developmentally critical windows of p20 to p60 and beyond. This methodology involves a manufacturing process for the printing and construction of a micro-drive implant, a surgical implantation procedure, and a post-surgery recovery strategy, all of which are specifically tailored for use in juvenile mice. Several considerations were influential in the development of this protocol, including the small size and relative weakness of juvenile mice compared to their adult counterparts, as well as the reduced ossification of the juvenile mouse skull onto which the micro-drive needed to be attached.
Two primary methods commonly used to perform in vivo electrophysiology are arrays of electrodes (e.g., tetrodes) and silicon probes. Silicon probes are lightweight, can provide a large number of recording sites per unit weight, and have been previously utilized in juvenile rats25. However, silicon probes are relatively expensive per unit. In contrast, the micro-drive described in this manuscript can be constructed using less than $50 USD in raw materials, making it a cost-effective option for in vivo recording. In addition, silicon probes must often be implanted in fixed lines, which prohibits the recording of spatially diverse brain regions. In contrast, the micro-drive design described in this manuscript utilizes independently adjustable tetrodes to accommodate simultaneous recordings in up to 16 different locations with virtually no restriction on the spatial relationship between those locations. This micro-drive design can be easily modified to allow for targeting different locations than those described here by moving the cannula hole extrusions to any desired anterior/posterior and medial/distal location. When targeting alternate brain areas, it is important to note that while the tetrodes will often travel straight, it is possible for these thin wires to deflect slightly as they exit the micro-drive cannula. Thus, the smaller or more ventral a brain region is, the more challenging it will be to successfully target the area with tetrodes.
The micro-drive implant described in this manuscript is foundationally similar to several prior tetrode-based micro-drive designs23,32,33,34,35 in that the individual tetrodes are affixed to screws, which allow for the fine control of the recording depth of each tetrode. While several features of the current micro-drive design are unique, including the ease of targeting spatially distributed brain areas, the primary novelty of the current manuscript is the description of surgical implantation and post-surgery recovery strategies, which allow for chronic studies of network activity in still-developing juvenile mice. Indeed, the surgery and recovery methodologies described here could be adapted to support other implants in juvenile mice.
To maintain a consistent recording across multiple days, the wires or probes must be rigidly affixed to the skull. While the overall structure of the mouse skull undergoes only minor changes after p20, the skull thickens considerably between ages p20 and p4536. Indeed, the skull at p20 is insufficiently rigid to support an attached implant without being damaged. To overcome this biological limitation, this protocol artificially thickens the skull via cyanoacrylate during the implantation surgery. Implantation in mice younger than p20 is likely possible using this strategy, but the mouse skull undergoes considerable size and shape changes until roughly p2036. Thus, implantation for extended periods in mice younger than p20 is not recommended as the cyanoacrylate and fixed bone screws in the still-developing skull may significantly impact the natural growth of the skull and the underlying brain tissue development. Importantly, in this study, no impact on the gross measurements of the skull or brain size was observed following chronic implantation starting at p20 (Figure 5C).
A critical step in the method described in this manuscript is the post-surgery recovery strategy; according to this strategy, the weight of the implant should be continuously counterbalanced as the mouse matures and undergoes muscular and musculoskeletal system development. Early after implantation, mice are unable to successfully bear the weight of the implant without the counterbalance, leading to malnourishment and dehydration as the mouse cannot adequately reach the food and water sources in its cage. The counterbalance system is easy and inexpensive to construct, trivial to implement, and allows mice of any implantable age to freely explore the entirety of their home cage, thus ensuring adequate nutrition and hydration. As mice age, the amount of counterbalance can be lessened until it can be entirely removed in adult mice; however, the continued use of the counterbalance system is recommended for the duration of the experiment with at least a nominal counterweight attached at all times. While an adult mouse may be able to bear the size and weight of the micro-drive over time, continued natural movement during free behavior with no ameliorating counterweight produces torque and shearing force on the bone screws that anchor the micro-drive onto the skull, making it increasingly likely to become detached, especially during longer chronic experiments.
Two important limitations are of note for the current study. First, to assess the impact of implantation at p20 on skull and brain development, several cohorts of mice were sacrificed after prolonged implantation (Figure 5C). While these analyses revealed no significant impact of implantation on the skull cavity size or brain mass (Figure 5C), the current study did not examine the skull size or brain mass at multiple time points throughout the early developmental period of p20-p60. While prior work demonstrates that the development of the brain cavity is completed by p2036, it is possible that implantation at this early window may produce unanticipated changes that are corrected or compensated for by the adult ages that were evaluated here. Second, the experiments that produced the electrophysiological data shown in Figure 3 and Figure 4 were not designed to maximize the cell yield. Thus, while the data presented here demonstrate stable, chronic recordings and well-isolated single units, they should not be taken as representative of the maximal potential yield for this device.
Many human neurological and psychiatric disorders manifest during periods of early development or across adolescence, including autism and schizophrenia. However, little is known regarding the circuit-level dysfunction that may underlie these diseases, despite the plethora of mouse models available. The identification of these initial network changes is critical for creating early detection strategies and treatment paradigms. Yet, due to technical challenges, it remains unclear how network function is disrupted across development in mouse models of neuropsychiatric diseases. The micro-drive and recovery strategy described here is designed to support investigations into multi-regional brain network development in the mouse brain and, thus, allow researchers to measure healthy brain development as well as identify alterations to that development in mouse models of disease.

The brain undergoes large-scale changes during the critical developmental windows of childhood and adolescence1,2,3. Many neurologic and psychiatric diseases, including autism and schizophrenia, first manifest behaviorally and biologically during this period of juvenile and adolescent brain development4,5,6. While much is known regarding the cellular, synaptic, and genetic changes that occur across early development, comparatively little is known regarding how circuit- or network-level processes change throughout this time window. Importantly, circuit-level brain function, which ultimately underlies complex behaviors, memory, and cognition, is a non-predictable, emergent property of cellular and synaptic function7,8,9,10. Thus, to fully understand network-level brain function, it is necessary to directly study neural activity at the level of an intact neural circuit. In addition, to identify how brain activity is altered throughout the progression of neuropsychiatric disorders, it is critical to examine network activity in a valid disease model during the specific temporal window when the behavioral phenotypes of the disease manifest and to track the observed changes as they persist into adulthood.
One of the most common and powerful scientific model organisms is the mouse, with large numbers of unique genetic strains that model neurodevelopmental disorders with age-dependent onset of the behavioral and/or mnemonic phenotypes11,12,13,14,15,16,17,18,19,20,21. While it is challenging to correlate precise developmental time points between the brains of humans and mice, morphological and behavioral comparisons indicate that p20-p21 mice represent the human ages of 2-3 years old, and p25-p35 mice represent the human ages of 11-14 years old, with mice likely reaching the developmental equivalent of a human 20 year old adult by p603,22. Thus, to better understand how the juvenile brain develops and to identify how the neural networks of the brain become dysfunctional in diseases like autism or schizophrenia, it would be ideal to directly monitor brain activity in vivo in mice across the ages of 20 days to 60 days old.
However, a fundamental challenge in monitoring brain activity across early development in mice is the small size and relative weakness of juvenile mice. The chronic implantation of electrodes, which is necessary for longitudinal studies of brain development, typically requires large, bulky housing to protect the fine electrode wires and interface boards23,24, and the implants must be firmly attached to the mouse skull, which is thinner and less rigid in young mice due to reduced ossification. Thus, virtually all studies of in vivo rodent physiology have been performed in adult subjects due to their relative size, strength, and skull thickness. To date, most studies exploring in vivo juvenile rodent brain physiology have been performed in wild-type juvenile rats, which necessarily limits the ability to experimentally monitor juvenile brain function in a freely behaving model of a human disorder25,26,27,28,29,30.
This manuscript describes novel implant housing, a surgical implantation procedure, and a post-surgery recovery strategy to chronically study the long-term (up to 4 or more weeks) in vivo brain function of juvenile mice across a developmentally critical time window (p20 to p60 and beyond). The implantation procedure allows for the reliable, permanent affixation of the electrodes to the skulls of juvenile mice. Furthermore, the micro-drive design is lightweight, as this micro-drive weighs ~4-6 g when fully assembled, and due to the minimal counterbalancing required to offset the weight of the implant, it does not impact the behavioral performance of juvenile mice during typical behavioral paradigms.

<table><tbody><tr><td>10 V video tracking LEDs</td><td>Neuralynx</td><td>HS-LED-Red/Green-omni-10V</td><td>For use with headstage pre-amplifiers that contain LED sockets for movement tracking purposes</td></tr><tr><td>16TT EIB Board</td><td>Neuralynx</td><td>EIB-36-16TT</td><td>Electronic interface board- omnetics connector</td></tr><tr><td>16TT headstage pre-amplifier</td><td>Neuralynx</td><td>HS-36-LED</td><td>Omnetics 44 socket signal amplifier between EIB board and tether cable for recording applications; includes connectors for headstage LEDs for movement tracking purposes</td></tr><tr><td>Baby-Mixter hemostat</td><td>FST</td><td>13013-14</td><td>Fine curved hemostat</td></tr><tr><td>Bone anchor screw</td><td>Stoelting</td><td>51457</td><td>Used to attach EIB board to main drive body</td></tr><tr><td>Burpenorphine</td><td>ZooPharm</td><td>Lot #BERLAB0.5-221207</td><td>Burpenorphine (0.5 mg/mL) 5mL quantity</td></tr><tr><td>Cable tether</td><td>Neuralynx</td><td>HS-36 Litz Tether</td><td>Lightweight shielded wire tether for omnetics headstages; length options of 1 m/2 m/3 m/5 m</td></tr><tr><td>Carprofen/Rimadyl</td><td>Bio-Serve</td><td>MD150-2</td><td>Post-operative anti-inflammatory agent</td></tr><tr><td>Clear resin v4</td><td>Formlabs</td><td>FLGPGR04</td><td>Liquid resin that is photopolymerized by 3D printer during the 3D printing process</td></tr><tr><td>Custom (shuttle) screw</td><td>Advanced Machining and Tooling, Inc.</td><td>Custom</td><td>Machined and threaded custom screws</td></tr><tr><td>Dental acrylic liquid component</td><td>Teets denture material</td><td>Lot# 329801</td><td>liquid&amp;nbsp; component of denture material (see above)</td></tr><tr><td>Dental acrylic powder component</td><td>Teets denture material</td><td>Lot# 583987</td><td>&quot;cold cure&quot; denture material, methyl methacrylate; mixed with liquid component for application to secure recording device in place</td></tr><tr><td>DietGel Boost</td><td>ClearH2O</td><td>72-04-5022</td><td>High calorie dietary supplement for young/recovering mice</td></tr><tr><td>Digital Lynx 16SX</td><td>Neuralynx</td><td>DigitalLynx 16SX Base</td><td>Main recording apparatus with 16 combo board slots for up to 512 recording channels</td></tr><tr><td>Dissector scissors- heavy blades</td><td>FST</td><td>14082-09</td><td>Various</td></tr><tr><td>Dumont #5 ceramic coated forceps</td><td>FST</td><td>11252-50</td><td>Tetrode handling/threading/pinning</td></tr><tr><td>Dumont #5SF forceps</td><td>FST</td><td>11252-00</td><td>Multipurpose assembly use</td></tr><tr><td>Dumont #5SF forceps</td><td>FST</td><td>11252-00</td><td>Multipurpose surgical use</td></tr><tr><td>Dumont #7 fine forceps (curved)</td><td>FST</td><td>11274-20</td><td>Various</td></tr><tr><td>Dumont #7 fine forceps (curved)</td><td>FST</td><td>11274-20</td><td>Multipurpose surgical use</td></tr><tr><td>EIB-36 plating adapter</td><td>Neuralynx</td><td>EIB-36 plating adapter</td><td>Plating/assembly use</td></tr><tr><td>EIB-36 plating adapter</td><td>Neuralynx</td><td>EIB-36 plating adapter</td><td>Stereotactic accessory for lowering drive onto skull during surgery</td></tr><tr><td>Euthasol</td><td>Virbac</td><td>710101</td><td>Pentobarbital sodium for euthanasia</td></tr><tr><td>Extra fine Bonn scissors</td><td>FST</td><td>14083-38</td><td>Various</td></tr><tr><td>Extra fine graefe forceps</td><td>FST</td><td>11150-10</td><td>Small straight serrated forceps</td></tr><tr><td>Extra fine graefe forceps</td><td>FST</td><td>11150-10</td><td>Small straight serrated forceps</td></tr><tr><td>Fine hemostats</td><td>FST</td><td>13006-12</td><td>Fine hemostats</td></tr><tr><td>Fine scissors- CeramaCut</td><td>FST</td><td>14958-09</td><td>Tetrode cutting</td></tr><tr><td>Fine scissors- ToughCut</td><td>FST</td><td>14058-09</td><td>Various</td></tr><tr><td>Form 3+</td><td>Formlabs</td><td>PKG-F3-P-WS-SVC-BASIC</td><td>3D printer for fabrication of all printed parts/materials; low-force stereolithography 3D printer (LFS)</td></tr><tr><td>Gel super glue</td><td>Loctite</td><td>1363589</td><td>Various steps</td></tr><tr><td>Graefe forceps</td><td>FST</td><td>11049-10</td><td>Small angled serrated forceps</td></tr><tr><td>Ground wire</td><td>A-M Systems</td><td>Lot# 582335</td><td>Stainless steel bare wire, .005&quot; diameter, annealed, 100 feet</td></tr><tr><td>Hair removal gel</td><td>Generic</td><td>Commercially available</td><td>For pre-op removal of hair from top of mouse head</td></tr><tr><td>Heat gun</td><td>Dewalt</td><td>D26960K</td><td>Tetrode fusion following spinning</td></tr><tr><td>High temperature cautery kit</td><td>FST</td><td>18010-00</td><td>For use with bone wax if applicable</td></tr><tr><td>Hot bead sterilizer</td><td>FST</td><td>18000-45</td><td>Electrical sterilization apparatus for ad hoc instrument sterilization during surgical procedures</td></tr><tr><td>Isoflurane</td><td>Covetrus</td><td>11695067771</td><td>Standard isoflurane liquid anesthsia for use in isoflurane vaporizer to max 5%</td></tr><tr><td>Isopropyl alcohol 91%</td><td>Generic</td><td>Commercially available</td><td>For standard pre-operative sterilization procedure</td></tr><tr><td>Jewelry screw (bone screws for juvenile mice)</td><td>Component supply co.</td><td>MX-000120-02SFL</td><td>S/S machine screw #000-120 x 1/8&#039;&#039; filister head, slotted drive</td></tr><tr><td>LaGrange scissors</td><td>FST</td><td>14173-12</td><td>Various</td></tr><tr><td>Large polyimide tubing</td><td>Nordson medical</td><td>Lot # 13564</td><td>Polyimide tubing- inner diameter 0.0071&quot;; outer diameter 0.0115&quot;; length 36&quot;</td></tr><tr><td>Liquid super glue</td><td>Loctite</td><td>1365882</td><td>Various steps</td></tr><tr><td>Micro drill</td><td>Foredom</td><td>K.1070</td><td>K.1070 high speed rotary micromotor kit; with control box, 3/32&quot; collet, variable speed foot control, handpiece cradle; stereotactically fittable; 100&amp;ndash;115 V use</td></tr><tr><td>Micro drill burr (0.5 mm+)</td><td>FST</td><td>19007-05/07/09</td><td>Craniotomy</td></tr><tr><td>Mineral oil</td><td>Sigma</td><td>Pcode 1002076577; M5904-500mL</td><td>Various steps</td></tr><tr><td>Mineral oil</td><td>Sigma</td><td>Pcode 1002076577; M5904-500mL</td><td>For use keeping craniotomy holes open</td></tr><tr><td>Miniature flathead screwdriver</td><td>FST</td><td>30051-10</td><td>Insertion/tightening of bone screws</td></tr><tr><td>Neosporin Triple Antibiotic Ointment</td><td>Johnson &amp;amp; Johnson</td><td>512373700</td><td>Antibiotic ointment</td></tr><tr><td>Omnetics 44 socket nano connector</td><td>Neuralynx</td><td>Neuralynx part #A70427-801</td><td>NONSTANDARD ITEM- omnetics 44 socket (female) dual row straight leg nano connector with 2 guide pins (male) for use with custom-made counterbalance apparatus</td></tr><tr><td>Platinum 10% iridium wire</td><td>California fine wire</td><td>MO# M374710</td><td>Fine recording wire spun into tetrodes for use during recording by use of the terode assembly station and spinner 2.0 (see below); HML NATRL VG BOND COAT; SIZE .0007 X 200FT</td></tr><tr><td>Platinum black plating solution</td><td>Neuralynx</td><td>Platinum black plating solution</td><td>Plating</td></tr><tr><td>Polycarbonate cage bottom</td><td>Thomas Scientific/Maryland plastics</td><td>1113M35; mfr. No. E0270</td><td>Standard cage bottom; can be fitted with wire mesh apparatus over top that contains chow+water bottle for unimplanted mice</td></tr><tr><td>Polycarbonate cage top with N10 micro filter</td><td>Ancare</td><td>N/A</td><td>Standard cage top to be modified with PVC pipe for counterbalance apparatus</td></tr><tr><td>Povidone iodine 10%</td><td>Generic</td><td>Commercially available</td><td>For standard pre-operative sterilization procedure</td></tr><tr><td>PVC pipe</td><td>Charlotte pipe</td><td>N/A</td><td>1/2&quot; x 600 PSI schedule 40 white PVC pipe; for use/assembly into counterbalance apparatus during mouse recovery</td></tr><tr><td>Scalpel blades- #4</td><td>FST</td><td>10060-00</td><td>Incision use</td></tr><tr><td>Scalpel handle- #4 gross anatomy</td><td>FST</td><td>10060-13</td><td>Incision use</td></tr><tr><td>Self-holding pin and bone screw forceps</td><td>FST</td><td>26100-00</td><td>Holder for bone and ground screws while inserting into skull</td></tr><tr><td>Small EIB pins</td><td>Neuralynx</td><td>Small EIB pins</td><td>Attachment of tetrode wires to EIB board</td></tr><tr><td>Small polyimide tubing</td><td>Nordson medical</td><td>Lot # 19102423</td><td>Polyimide tubing- inner diameter 0.004&#039;&#039;; outer diameter 0.0044&#039;&#039;; length 36&quot;</td></tr><tr><td>SolidWorks</td><td>Dassault Systemes</td><td>SolidWorks</td><td>3D CAD program for micro-drive design</td></tr><tr><td>Spatula and probe</td><td>FST</td><td>1090-13</td><td>Applicator for petroleum jelly/mineral oil + optional use for ad hoc tetrode straightening</td></tr><tr><td>Spring scissors- 8 mm</td><td>FST</td><td>15024-10</td><td>Scissors for cranial tissue incisions</td></tr><tr><td>Spring scissors- 8 mm</td><td>FST</td><td>15024-10</td><td>Initial incisions</td></tr><tr><td>Standard pattern forceps</td><td>FST</td><td>11000-12</td><td>Large serrated forceps</td></tr><tr><td>Surgical scissors- sharp-blunt</td><td>FST</td><td>14001-12</td><td>Various</td></tr><tr><td>Surgical scissors- ToughCut</td><td>FST</td><td>14054-13</td><td>Various</td></tr><tr><td>Tetrode assembly station</td><td>Neuralynx</td><td>Tetrode assembly station</td><td>Tetrode Assembly</td></tr><tr><td>Tetrode spinner 2.0</td><td>Neuralynx</td><td>Tetrode spinner 2.0</td><td>Tetrode Assembly</td></tr><tr><td>Two-part epoxy</td><td>Gorilla brand</td><td>4200102</td><td>Various steps</td></tr></tbody></table>

a lightweight drive implant for chronic tetrode recordings in juvenile mice

In vivo electrophysiology provides unparalleled insight into the sub-second-level circuit dynamics of the intact brain and represents a method of particular importance for studying mouse models of human neuropsychiatric disorders. However, such methods often require large cranial implants, which cannot be used in mice at early developmental time points. As such, virtually no studies of in vivo physiology have been performed in freely behaving infant or juvenile mice, despite the fact that a better understanding of neurological development in this critical window would likely provide unique insights into age-dependent developmental disorders such as autism or schizophrenia. Here, a micro-drive design, surgical implantation procedure, and post-surgery recovery strategy are described that allow for chronic field and single-unit recordings from multiple brain regions simultaneously in mice as they age from postnatal day 20 (p20) to postnatal day 60 (p60) and beyond, a time window roughly corresponding to the human ages of 2 years old through to adulthood. The number of recording electrodes and final recording sites can be easily modified and expanded, thus allowing flexible experimental control of the in vivo monitoring of behavior- or disease-relevant brain regions across development.

The above-described protocol was used to record local field potential signals and single units from multiple brain areas simultaneously in mice, with daily recordings conducted in the same mice from p20 to p60. Reported here are representative electrophysiological recordings from two mice and post-experiment histology demonstrating the final recording locations.
Surgical implantation of the micro-drive into p20 mice 
A micro-drive (Figure 1) was constructed (Figure 2) and surgically implanted into a p20 mouse, as described above. Immediately following the surgery, the mouse was attached to the counterbalance system (Figure 2G-I) and allowed to recover. Once the mouse was fully mobile, the micro-drive was plugged into an in vivo electrophysiology recording system. The cables connecting the micro-drive to the recording equipment were suspended above the mouse. Electrophysiological recordings (32 kHz) were obtained across all the channels for 1 h while the mouse behaved naturally in its home cage. Following the recording, the mouse was unplugged from the recording system, reattached to the counterbalance system, and returned to the vivarium with free access to water and chow.
Daily recording of neural activity 
Electrophysiological recordings were obtained daily for several weeks to enable the chronic monitoring of the same brain region across the critical developmental windows of p20-p60. Sample raw local field potentials (LFP) from across the chronic recordings are shown in Figure 3A,C. Isolated single units were simultaneously obtained from multiple tetrodes (Figure 3B). Units with similar waveforms were identified across multiple days (Figure 3B,&#160;middle and right), but due to the potential drift of the recording electrode, it was not possible to definitively claim that the same unit was being identified across days. In a separate mouse implanted at p20 and recorded daily for several weeks, neural activity was examined on a tetrode targeting dorsal area CA1. Large-amplitude ripples and well-isolated single units were identified on each day of the recording (Figure 4).These data indicate that stable, high-quality in vivo electrophysiological recordings could be from the same mouse across early development.
Histological confirmation of the recording sites and the developmental impact of chronic implantation 
Following the final recording day, the mouse was thoroughly anesthetized via isoflurane anesthesia followed by a lethal injection of pentobarbital sodium, and a current was passed through the electrode tips to produce small lesions at the recording sites. Post-experiment histological sectioning of the mouse brain allowed the visualization of the final recording sites (Figure 5A,B). In a separate cohort, three male and three female mice were surgically implanted at p20 as described above. Equal numbers of littermates were left unimplanted and maintained in identical housing conditions. The mice were sacrificed at p62 (6 weeks post-surgery for the implanted cohort). The skulls were carefully cleaned, and external measurements were taken of the bregma-to-lambda distance (Figure 5C,&#160;top left) and external maximal skull width at lambda (Figure 5C, top right). An incision was made along the midline of the skull, and one-half of the skull was removed to excise the brain for mass measurement (Figure 5C, bottom right). The height of the skull cavity at bregma was measured from the intact skull half (Figure 5C, bottom left). No measure was significantly different between the implanted and unimplanted cohorts (Wilcoxon rank-sum test), indicating that long-term implantation, starting at p20, has no gross impact on the natural development of the skull or brain volume.
<img alt="Figure 1" class="xfigimg" src="/files/ftp_upload/65228/65228fig01.jpg" /> 
Figure 1: Micro-drive components. Three-dimensional renderings of the (A) micro-drive body, (B) cannula, (C) cone, (D) lid, (E) screw attachments, and (F) tetrode-advancing screw. The critical features of each component are indicated. Measurement details can be extracted from the model files available at&#160;https://github.com/Brad-E-Pfeiffer/JuvenileMouseMicroDrive/. <a href="https://www.jove.com/files/ftp_upload/65228/65228fig01large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 2" class="xfigimg" src="/files/ftp_upload/65228/65228fig02.jpg" /> 
Figure 2: Micro-drive construction. (A) Side and (B) top view of the tetrode-advancing screw with the top and bottom screw attachments connected. (C) Side and (D) top view of the micro-drive with the body and cannula attached and the large polyimide tubing running through each cannula hole and trimmed to the bottom of the cannula. (E) Side view of the micro-drive with the screws and small polyimide tubing in place. The tops of the small polyimide tubes are trimmed immediately prior to tetrode loading. (F) Completed micro-drive attached to the stereotaxic apparatus. The protective cone that would normally surround the micro-drive has been removed for visualization purposes. Note that some of the screw attachments were printed in a black resin for this micro-drive. (G) Counterbalance support system. (H)Side and (I) top view of a mouse cage with the counterbalance support system attached. <a href="https://www.jove.com/files/ftp_upload/65228/65228fig02large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 3" class="xfigimg" src="/files/ftp_upload/65228/65228fig03.jpg" /> 
Figure 3: Representative electrophysiological recordings. A p20 mouse was implanted with a micro-drive as described above. Starting on p21 and every day thereafter for 2 weeks, the mouse was attached to the recording apparatus, and neural activity was recorded for at least 1 h. (A)&#160;Raw local field potential (LFP) recordings from the bilateral (L = left; R = right) anterior cingulate cortex (ACC), hippocampal area CA3 (CA3), and hippocampal area CA1 (CA1). The data were collected every day; for clarity, only data from odd days are displayed. All traces were taken during periods of immobility in the home cage. Scale bar: 1 mV, 2 s. (B) Representative single units isolated from hippocampal area CA3 (left) and CA1 (right) for the recordings in panel A. All the raw waveforms on each electrode are shown in black; the average is in red. Scale bar: 50 &#181;V, 0.2 ms. (C) Representative raw LFP traces for every 10th day until the final recording day at p60 for a second mouse implanted at p20. The data were collected every day; for clarity, only data from every 10th day are displayed. All the traces were taken during periods of immobility in the home cage. Scale bar: 1 mV, 2 s. <a href="https://www.jove.com/files/ftp_upload/65228/65228fig03large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 4" class="xfigimg" src="/files/ftp_upload/65228/65228fig04.jpg" /> 
Figure 4. Stability of the chronic recordings. A p20 mouse was implanted with a micro-drive, as described above. Starting on p21 and thereafter for 4 weeks, the mouse was attached to the recording apparatus, and neural activity was recorded for at least 1 h. Shown are data from the tetrodes targeting dorsal hippocampal CA1. (A) Raw (top) and ripple-filtered (bottom) LFP for identified ripple events at p21, p30, and p40. To identify ripple events, the raw LFP was band-pass filtered between 125 Hz and 300 Hz, and the ripple events were identified as transient increases in the ripple-band power greater than 3 standard deviations above the mean. The start and end of each ripple were defined as the point when the ripple band power returned to the mean. The identified ripples are shown in red. Scale bar: 100 ms, top-to-bottom: 1,000 &#181;V, 140 &#181;V, 1,800 &#181;V, 180 &#181;V, 9,000 &#181;V, 1,200 &#181;V, 10,000 &#181;V, 1,000 &#181;V. (B) A representative single unit from each day from the CA1-targeted tetrode for the recordings in panel A. All raw waveforms on each electrode are shown in black; the average is in red. Scale bar 0.2 ms, top-to-bottom: 50 &#181;V, 100 &#181;V, 100 &#181;V. (C)&#160;Autocorrelogram of all spikes for single units in panel B. These data demonstrate stable electrode placement within the hippocampal pyramidal layer across several weeks. <a href="https://www.jove.com/files/ftp_upload/65228/65228fig04large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 5" class="xfigimg" src="/files/ftp_upload/65228/65228fig05.jpg" /> 
Figure 5: Representative histology and impact on skull development. A p20 mouse was implanted with a micro-drive, as described above. Following the final recording day on p60, electrolytic lesions were produced at the recording sites, and the brain was perfused with 4% paraformaldehyde. To identify the recording sites, 50 &#181;m sections were produced. (A) Lesions in CA1 and CA3 of the hippocampus. The arrowhead denotes the CA3 recording site; the double-arrowhead denotes the CA1 recording site. Scale bar: 0.5 mm. (B) Lesions in the bilateral ACC. The arrowheads denote the ACC recording sites. Scale bar: 0.5 mm. (C) Skull size and brain mass measurements of p62 mice implanted with a micro-drive at p20 (gray) and unimplanted littermates (white). The p-value of the Wilcoxon rank-sum test is reported for each measurement. <a href="https://www.jove.com/files/ftp_upload/65228/65228fig05large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>

Watch this Scientific Journal Video about A Lightweight Drive Implant for Chronic Tetrode Recordings in Juvenile Mice at JoVE.com

Author Spotlight: A Lightweight Drive Implant for Chronic Tetrode Recordings in Juvenile Mice  

Here, we describe a micro-drive design, surgical implantation procedure, and post-surgery recovery strategy that allow for chronic field and single-unit recordings from multiple brain regions simultaneously in juvenile and adolescent mice across a critical developmental window from postnatal day 20 (p20) to postnatal day 60 (p60) and beyond.

A Lightweight Drive Implant for Chronic Tetrode Recordings in Juvenile Mice

In vivo electrophysiology provides unparalleled insight into the sub-second-level circuit dynamics of the intact brain and represents a method of ...

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Neuroscience

2.3K Views.  UT Southwestern Medical Center. Here, we describe a micro-drive design, surgical implantation procedure, and post-surgery recovery strategy that allow for chronic field and single-unit recordings from multiple brain regions simultaneously in juvenile and adolescent mice across a critical developmental window from postnatal day 20 (p20) to postnatal day 60 (p60) and beyond.

Video: Author Spotlight: A Lightweight Drive Implant for Chronic Tetrode Recordings in Juvenile Mice  

Construction of Microdrive Arrays for Chronic Neural Recordings in Awake Behaving Mice

State-of-the-art electrophysiological recordings from the brains of freely behaving animals allow researchers to simultaneously examine local field potentials (LFPs) from populations of neurons and action potentials from individual cells, as the animal engages in experimentally relevant tasks. Chronically implanted microdrives allow for brain recordings to last over periods of several weeks. Miniaturized drives and lightweight components allow for these long-term recordings to occur in small mammals, such as mice. By using tetrodes, which consist of tightly braided bundles of four electrodes in which each wire has a diameter of 12.5 &mu;m, it is possible to isolate physiologically active neurons in superficial brain regions such as the cerebral cortex, dorsal hippocampus, and subiculum, as well as deeper regions such as the striatum and the amygdala. Moreover, this technique insures stable, high-fidelity neural recordings as the animal is challenged with a variety of behavioral tasks. This manuscript describes several techniques that have been optimized to record from the mouse brain. First, we show how to fabricate tetrodes, load them into driveable tubes, and gold-plate their tips in order to reduce their impedance from M&Omega; to K&Omega; range. Second, we show how to construct a custom microdrive assembly for carrying and moving the tetrodes vertically, with the use of inexpensive materials. Third, we show the steps for assembling a commercially available microdrive (Neuralynx VersaDrive) that is designed to carry independently movable tetrodes. Finally, we present representative results of local field potentials and single-unit signals obtained in the dorsal subiculum of mice. These techniques can be easily modified to accommodate different types of electrode arrays and recording schemes in the mouse brain.

The design and assembly of microdrives for in vivo electrophysiological recordings of brain signals from the mouse is described. By attaching microelectrode bundles to sturdy driveable carriers, these techniques allow for long-term and stable neural recordings. The lightweight design allows for unrestricted behavioral performance by the animal following drive implantation.

State-of-the-art electrophysiological recordings from the brains of freely behaving animals allow researchers to simultaneously examine local field ...

Behavior

Micro-drive Array for Chronic in vivo Recording: Drive Fabrication

Chronic recording of large populations of neurons is a valuable technique for studying the function of neuronal circuits in awake behaving rats. Lightweight recording devices carrying a high density array of tetrodes allow for the simultaneous monitoring of the activity of tens to hundreds of individual neurons. Here we describe a protocol for the fabrication of a micro-drive array with twenty one independently movable micro-drives. This device has been used successfully to record from hippocampal and cortical neurons in our lab. We show how to prepare a custom designed, 3-D printed plastic base that will hold the micro-drives. We demonstrate how to construct the individual micro-drives and how to assemble the complete micro-drive array. Further preparation of the drive array for surgical implantation, such as the fabrication of tetrodes, loading of tetrodes into the drive array and gold-plating, is covered in a subsequent video article.

Micro-drive Array for Chronic in vivo Recording: Drive Fabrication

In this protocol we demonstrate how to fabricate a micro-drive array for chronic electrophysiological recordings in rats.

Chronic recording of large populations of neurons is a valuable technique for studying the function of neuronal circuits in awake behaving rats. ...

Biology

Micro-drive Array for Chronic in vivo Recording: Tetrode Assembly

The tetrode, a bundle of four electrodes, has proven to be a valuable tool for the simultaneous recording of multiple neurons in-vivo.  The differential amplitude of action potential signatures over the channels of a tetrode allows for the isolation of single-unit activity from multi-unit signals.  The ability to precisely control the stereotaxic location and depth of the tetrode is critical for studying coordinated neural activity across brain regions.  In combination with a micro-drive array, it is possible to achieve precise placement and stable control of many tetrodes over the course of days to weeks.  In this protocol, we demonstrate how to fabricate and condition tetrodes using basic tools and materials, install the tetrodes into a multi-drive tetrode array for chronic in-vivo recording in the rat, make ground wire connections to the micro-drive array, and attach a protective cone onto the micro-drive array in order to protect the tetrodes from physical contact with the environment.



Micro-drive Array for Chronic in vivo Recording: Tetrode Assembly

In this protocol we demonstrate how to fabricate and condition tetrodes for use with a micro-drive array, which was designed for chronic electrophysiological recordings in rats. In addition, we illustrate the final stages of micro-drive array construction, which includes installing ground wires and a protective cone.

The tetrode, a bundle of four electrodes, has proven to be a valuable tool for the simultaneous recording of multiple neurons in-vivo.  The differential ...

Design and Assembly of an Ultra-light Motorized Microdrive for Chronic Neural Recordings in Small Animals

The ability to chronically record from populations of neurons in freely behaving animals has proven an invaluable tool for dissecting the function of neural circuits underlying a variety of natural behaviors, including navigation1 , decision making 2,3, and the generation of complex motor sequences4,5,6. Advances in precision machining has allowed for the fabrication of light-weight devices suitable for chronic recordings in small animals, such as mice and songbirds. The ability to adjust the electrode position with small remotely controlled motors has further increased the recording yield in various behavioral contexts by reducing animal handling.6,7
 Here we describe a protocol to build an ultra-light motorized microdrive for long-term chronic recordings in small animals. Our design evolved from an earlier published version7, and has been adapted for ease-of use and cost-effectiveness to be more practical and accessible to a wide array of researchers. This proven design 8,9,10,11 allows for fine, remote positioning of electrodes over a range of ~ 5 mm and weighs less than 750 mg when fully assembled. We present the complete protocol for how to build and assemble these drives, including 3D CAD drawings for all custom microdrive components.

The design, fabrication and assembly of an ultra-light motorized microdrive is described. The device provides a cost-effective and easy-to-use solution for chronic recordings of single units in small behaving animals.

The ability to chronically record from populations of neurons in freely behaving animals has proven an invaluable tool for dissecting the function of ...

Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices

Astrocytes form together with neurons tripartite synapses, where they integrate and modulate neuronal activity. Indeed, astrocytes sense neuronal inputs through activation of their ion channels and neurotransmitter receptors, and process information in part through activity-dependent release of gliotransmitters. Furthermore, astrocytes constitute the main uptake system for glutamate, contribute to potassium spatial buffering, as well as to GABA clearance. These cells therefore constantly monitor synaptic activity, and are thereby sensitive indicators for alterations in synaptically-released glutamate, GABA and extracellular potassium levels. Additionally, alterations in astroglial uptake activity or buffering capacity can have severe effects on neuronal functions, and might be overlooked when characterizing physiopathological situations or knockout mice. Dual recording of neuronal and astroglial activities is therefore an important method to study alterations in synaptic strength associated to concomitant changes in astroglial uptake and buffering capacities. Here we describe how to prepare hippocampal slices, how to identify stratum radiatum astrocytes, and how to record simultaneously neuronal and astroglial electrophysiological responses. Furthermore, we describe how to isolate pharmacologically the synaptically-evoked astroglial currents.

The preparation of acute brain slices from isolated hippocampi, as well as the simultaneous electrophysiological recordings of astrocytes and neurons in stratum radiatum during stimulation of schaffer collaterals is described. The pharmacological isolation of astroglial potassium and glutamate transporter currents is demonstrated.

Astrocytes form together with neurons tripartite synapses, where they integrate and modulate neuronal activity. Indeed, astrocytes sense neuronal inputs ...

A Lightweight, Headphones-based System for Manipulating Auditory Feedback in Songbirds

Experimental manipulations of sensory feedback during complex behavior have provided valuable insights into the computations underlying motor control and sensorimotor plasticity1. Consistent sensory perturbations result in compensatory changes in motor output, reflecting changes in feedforward motor control that reduce the experienced feedback error. By quantifying how different sensory feedback errors affect human behavior, prior studies have explored how visual signals are used to recalibrate arm movements2,3 and auditory feedback is used to modify speech production4-7. The strength of this approach rests on the ability to mimic naturalistic errors in behavior, allowing the experimenter to observe how experienced errors in production are used to recalibrate motor output.
Songbirds provide an excellent animal model for investigating the neural basis of sensorimotor control and plasticity8,9. The songbird brain provides a well-defined circuit in which the areas necessary for song learning are spatially separated from those required for song production, and neural recording and lesion studies have made significant advances in understanding how different brain areas contribute to vocal behavior9-12. However, the lack of a naturalistic error-correction paradigm - in which a known acoustic parameter is perturbed by the experimenter and then corrected by the songbird - has made it difficult to understand the computations underlying vocal learning or how different elements of the neural circuit contribute to the correction of vocal errors13.
The technique described here gives the experimenter precise control over auditory feedback errors in singing birds, allowing the introduction of arbitrary sensory errors that can be used to drive vocal learning. Online sound-processing equipment is used to introduce a known perturbation to the acoustics of song, and a miniaturized headphones apparatus is used to replace a songbird's natural auditory feedback with the perturbed signal in real time. We have used this paradigm to perturb the fundamental frequency (pitch) of auditory feedback in adult songbirds, providing the first demonstration that adult birds maintain vocal performance using error correction14. The present protocol can be used to implement a wide range of sensory feedback perturbations (including but not limited to pitch shifts) to investigate the computational and neurophysiological basis of vocal learning.

We describe the design and assembly of miniaturized headphones suitable for replacing a songbird&#x2019;s natural auditory feedback with a manipulated acoustic signal. Online sound processing hardware is used to manipulate song output, introduce real-time errors in auditory feedback via the headphones, and drive vocal motor learning.

Experimental manipulations of sensory feedback during complex behavior have provided valuable insights into the computations underlying motor control and ...

Design and Fabrication of Ultralight Weight, Adjustable Multi-electrode Probes for Electrophysiological Recordings in Mice

The number of physiological investigations in the mouse, mus musculus, has experienced a recent surge, paralleling the growth in methods of genetic targeting for microcircuit dissection and disease modeling. The introduction of optogenetics, for example, has allowed for bidirectional manipulation of genetically-identified neurons, at an unprecedented temporal resolution. To capitalize on these tools and gain insight into dynamic interactions among brain microcircuits, it is essential that one has the ability to record from ensembles of neurons deep within the brain of this small rodent, in both head-fixed and freely behaving preparations. To record from deep structures and distinct cell layers requires a preparation that allows precise advancement of electrodes towards desired brain regions. To record neural ensembles, it is necessary that each electrode be independently movable, allowing the experimenter to resolve individual cells while leaving neighboring electrodes undisturbed. To do both in a freely behaving mouse requires an electrode drive that is lightweight, resilient, and highly customizable for targeting specific brain structures.
A technique for designing and fabricating miniature, ultralight weight, microdrive electrode arrays that are individually customizable and easily assembled from commercially available parts is presented. These devices are easily scalable and can be customized to the structure being targeted; it has been used successfully to record from thalamic and cortical regions in a freely behaving animal during natural behavior.

Understanding the neural substrates of behavior requires brain circuit ensemble recording. Because of its genetic tractability, the mouse offers a model for circuit dissection and disease mimicry. Here, a method of designing and fabricating miniaturized probes is described that is suitable for targeting deep brain structure in the mouse.

The number of physiological investigations in the mouse, mus musculus, has experienced a recent surge, paralleling the growth in methods of genetic ...

Hybrid Microdrive System with Recoverable Opto-Silicon Probe and Tetrode for Dual-Site High Density Recording in Freely Moving Mice

Multi-regional neural recordings can provide crucial information to understanding fine-timescale interactions between multiple brain regions. However, conventional microdrive designs often only allow use of one type of electrode to record from single or multiple regions, limiting the yield of single-unit or depth profile recordings. It also often limits the ability to combine electrode recordings with optogenetic tools to target pathway and/or cell type specific activity. Presented here is a hybrid microdrive array for freely moving mice to optimize yield and a description of its fabrication and reuse of the microdrive array. The current design employs nine tetrodes and one opto-silicon probe implanted in two different brain areas simultaneously in freely moving mice. The tetrodes and the opto-silicon probe are independently adjustable along the dorsoventral axis in the brain to maximize the yield of unit and oscillatory activities. This microdrive array also incorporates a set-up for light, mediating optogenetic manipulation to investigate the regional- or cell type-specific responses and functions of long-range neural circuits. In addition, the opto-silicon probe can be safely recovered and reused after each experiment. Because the microdrive array consists of 3D-printed parts, the design of microdrives can be easily modified to accommodate various settings. First described is the design of the microdrive array and how to attach the optical fiber to a silicon probe for optogenetics experiments, followed by fabrication of the tetrode bundle and implantation of the array into a mouse brain. The recording of local field potentials and unit spiking combined with optogenetic stimulation also demonstrate feasibility of the microdrive array system in freely moving mice.

This protocol describes the construction of a hybrid microdrive array that allows implantation of nine independently adjustable tetrodes and one adjustable opto-silicon probe in two brain regions in freely moving mice. Also demonstrated is a method for safely recovering and reusing the opto-silicon probe for multiple purposes.

Multi-regional neural recordings can provide crucial information to understanding fine-timescale interactions between multiple brain regions. However, ...

Open&#45;Source Implant for Multi&#45;Region Electrophysiological Monitoring in Rats

The TD Drive: A Parametric, Open-Source Implant for Multi&#45;Area Electrophysiological Recordings in Behaving and Sleeping Rats

Intricate interactions between multiple brain areas underlie most functions attributed to the brain. The process of learning, as well as the formation and consolidation of memories, are two examples that rely heavily on functional connectivity across the brain. In addition, investigating hemispheric similarities and/or differences goes hand in hand with these multi-area interactions. Electrophysiological studies trying to further elucidate these complex processes thus depend on recording brain activity at multiple locations simultaneously and often in a bilateral fashion. Presented here is a 3D-printable implant for rats, named TD Drive, capable of symmetric, bilateral wire electrode recordings, currently in up to ten distributed brain areas simultaneously. The open-source design was created employing parametric design principles, allowing prospective users to easily adapt the drive design to their needs by simply adjusting high-level parameters, such as anterior-posterior and mediolateral coordinates of the recording electrode locations. The implant design was validated in n = 20 Lister Hooded rats that performed different tasks. The implant was compatible with tethered sleep recordings and open field recordings (Object Exploration) as well as wireless recording in a large maze using two different commercial recording systems and headstages. Thus, presented here is the adaptable design and assembly of a new electrophysiological implant, facilitating fast preparation and implantation.

Author Spotlight: Deciphering Memory and Learning Through Neural Implants for Multi&#45;Region Brain Studies

Here, we present a unique, 3D-printable implant for rats, named TD Drive, capable of symmetric, bilateral wire electrode recordings, currently in up to ten distributed brain areas simultaneously.

Intricate interactions between multiple brain areas underlie most functions attributed to the brain. The process of learning, as well as the formation and ...

A Cost-Effective, Versatile Probe Implant System for Electrophysiology in Mice

The DREAM Implant: A Lightweight, Modular, and Cost-Effective Implant System for Chronic Electrophysiology in Head-Fixed and Freely Behaving Mice

Chronic electrophysiological recordings in rodents have significantly improved our understanding of neuronal dynamics and their behavioral relevance. However, current methods for chronically implanting probes present steep trade-offs between cost, ease of use, size, adaptability, and long-term stability.
This protocol introduces a novel chronic probe implant system for mice called the DREAM (Dynamic, Recoverable, Economical, Adaptable, and Modular), designed to overcome the trade-offs associated with currently available options. The system provides a lightweight, modular and cost-effective solution with standardized hardware elements that can be combined and implanted in straightforward steps and explanted safely for recovery and multiple reuse of probes, significantly reducing experimental costs.
The DREAM implant system integrates three hardware modules: (1) a microdrive that can carry all standard silicon probes, allowing experimenters to adjust recording depth across a travel distance of up to 7 mm; (2) a three-dimensional (3D)-printable, open-source design for a wearable Faraday cage covered in copper mesh for electrical shielding, impact protection, and connector placement, and (3) a miniaturized head-fixation system for improved animal welfare and ease of use. The corresponding surgery protocol was optimized for speed (total duration: 2 h), probe safety, and animal welfare.
The implants had minimal impact on animals&#39; behavioral repertoire, were easily applicable in freely moving and head-fixed contexts, and delivered clearly identifiable spike waveforms and healthy neuronal responses for weeks of post-implant data collection. Infections and other surgery complications were extremely rare.
As such, the DREAM implant system is a versatile, cost-effective solution for chronic electrophysiology in mice, enhancing animal well-being, and enabling more ethologically sound experiments. Its design simplifies experimental procedures across various research needs, increasing accessibility of chronic electrophysiology in rodents to a wide range of research labs.

Here, we introduce a lightweight, cost-effective probe implant system for chronic electrophysiology in rodents optimized for ease of use, probe recovery, experimental versatility, and compatibility with behavior.

Chronic electrophysiological recordings in rodents have significantly improved our understanding of neuronal dynamics and their behavioral relevance. ...

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The DREAM Implant: A Lightweight, Modular, and Cost-Effective Implant System for Chronic Electrophysiology in Head-Fixed and Freely Behaving Mice