Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate

1.1K Views

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10:32 min

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May 19th, 2023

DOI :

10.3791/65413-v

May 19th, 2023

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Lisa A. Krattiger¹, Maria Mitsi², Benjamin R. Simona², Martin Ehrbar¹

¹Department of Obstetrics, University Hospital Zurich, University of Zurich, ²Ectica Technologies AG

Transcript

Summary

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Vascularized Osteogenic Niche

Poly ethylene Glycol Hydrogels

In Vitro Vascularization

Bone Marrow derived Mesenchymal Stromal Cells

High throughput Compatible Models

Chapters in this video

0:00

Introduction

1:04

Establishing the 3D Stromal Monoculture

2:44

Establishing the 3D Stromal‐Endothelial Cell Co‐Culture

4:25

Quantifying the Endothelial Cell Network Formation after the 3D Stromal‐Endothelial Cell Co‐Culture

6:53

Assessing the Osteogenic Differentiation of 3D Stromal‐Endothelial Cells by Monitoring the Alkaline Phosphatase Activity

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