Simple, Affordable, and Modular Patterning of Cells using DNA

4.0K Views

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08:59 min

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February 24th, 2021

DOI :

10.3791/61937-v

February 24th, 2021

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Katelyn A. Cabral¹, David M. Patterson², Olivia J. Scheideler¹, Russell Cole³, Adam R. Abate⁴^,⁵^,⁶, David V. Schaffer⁷^,⁸, Lydia L. Sohn⁹, Zev J. Gartner²^,⁶^,¹⁰

Summary

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Cell cell Interactions

Universal Anchor Solution

Cell Suspension

Chapters in this video

0:05

Introduction

0:30

Photopattern DNA onto Aldehyde-Functionalized Slides

2:46

Lift and Label Cells with Cholesterol-Modified DNA

4:21

Pattern the DNA-labeled Cells and Transferring into Hydrogel for 3D Culture

7:22

Results: Adhesion of CMO-Labeled Cells to DNA Patterns

8:32

Conclusion

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