Isolation of Volatile Compounds from Pinus pinaster Seedlings Using Headspace Trapping with Packed Tubes

To begin, prepare a setup for trapping headspace volatiles with packed stainless steel traps. Place pine material set in covered glassware or inside PET bags to accumulate headspace volatiles. For a closed-loop system, connect the pump outlet to the PTFE tubing.

Then attach the PTFE tubing to activated charcoal air filters. Connect the air filters to additional tubing leading to the glassware or PET bags with the pine material. Next, attach the packed tube to the glassware or PET bag outlet and connect additional PTFE tubing from the stainless steel tube outlet to the mass flow pump inlet.

Using a mass flow pump, pump 100 liters of filtered air through the pine system at 0.6 liters per minute to collect volatiles. After collecting volatiles, close the packed tubes at both ends with airtight storage caps. Finally, rinse all PTFE tubing in glassware with 70%ethanol and heat in an oven at 230 degrees Celsius for two hours.

Volatile profiles of maritime pine in vitro shoots obtained through hydro distillation or distillation extraction were similar within the chemotype analyzed. Chemical profiles obtained through solid phase micro extraction techniques showed variations depending on the amount of plant material and volatiles trapped.