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Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

DOI :

10.3791/54289-v

August 6th, 2016

August 6th, 2016

17,220 Views

1Center for Innovation in Global Health Technologies (CIGHT), Department of Biomedical Engineering, Northwestern University, 2Pritzker School of Medicine, The University of Chicago

We describe here a simple and rapid paper-based DNA extraction method of HIV proviral DNA from whole blood detected by quantitative PCR. This protocol can be extended for use in detecting other genetic markers or using alternative amplification methods.

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