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Assessment of Cardiac Function and Energetics in Isolated Mouse Hearts Using 31P NMR Spectroscopy

DOI :

10.3791/2069-v

11:38 min

August 31st, 2010

August 31st, 2010

25,772 Views

1Department of Anesthesiology & Pain Medicine, University of Washington School of Medicine

Langendorff-mode isolated heart perfusion, in conjunction with 31P NMR spectroscopy, combines the fields of biochemistry and physiology into one experiment. The protocol allows for the dynamic measurement of high energy phosphate content and turnover in the heart while concurrently monitoring physiologic function. When performed correctly, this is a valuable technique in the assessment of cardiac energetics.

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Cardiac Function

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