Measurement of Cardiac Function and Sample Collection
7:17
Determination of Myocardial Glucose Oxidation Rate
8:48
Determination of Myocardial Oleate Oxidation Rates
10:14
Results: Measurements from Two Stressed Hearts
11:40
Conclusion
Transcript
The overall goal of this procedure is to obtain simultaneous real time measures of myocardial rates of glucose oxidation and fatty acid oxidation, as well as cardiac contractual function in an isolated working rat heart. This method can help answe
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The following protocol describes the preparation and utilization of buffers for the quantitative measurement of rates of glucose and fatty acid oxidation in the isolated working rat heart. The methods used for sample analysis and data interpretation are also discussed.