JoVE Logo
Faculty Resource Center

Sign In

Concept
Experiment

Fluorescein Staining in an Autoimmune Dry Eye Rat Model to Examine Corneal Damage


Transcript


Start with an anesthetized rat presenting symptoms of autoimmune dry eye disease.

Apply an appropriate amount of fluorescein dye over the rat's cornea.

Close and open the rat's eyelid to ensure the fluorescein spreads evenly over the cornea.

Allow the dye to settle and adhere to the cornea.

In autoimmune dry eye disease, the immune cells mistakenly target and attack the lacrimal glands, decreasing tear production.

This leads to the desiccation of the corneal epithelium and damage to the underlying Bowman's membrane — an acellular zone anterior to the stroma.

The fluorescein molecules permeate through the damaged corneal barriers to reach the underlying collagen-rich stromal layer.

Here, the fluorescein molecules bind to collagen, resulting in the staining of the collagen fibers.

Using an appropriate imaging technique, acquire images of the stained cornea.

Analyze the number, area, and intensity of the green fluorescent spots to measure the extent of corneal damage.

Usage Statistics

-- Views

Related Videos

article

Experimental Autoimmune Uveitis: An Intraocular Inflammatory Mouse Model

article

A Rabbit Model of Aqueous-Deficient Dry Eye Disease Induced by Concanavalin A Injection into the Lacrimal Glands: Application to Drug Efficacy Studies

article

Establishment of a Severe Dry Eye Model Using Complete Dacryoadenectomy in Rabbits

;
JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved