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Maintaining Wolbachia in Cell-free Medium

DOI :

10.3791/223-v

July 4th, 2007

July 4th, 2007

10,230 Views

1Malaria Research Institute, Bloomberg School of Public Health, Johns Hopkins University

This video describes a method for purifying Wolbachia pipientis from an Anopheles gambiae cell line and then culturing the endosymbiont in cell-free medium. An assay for viability of the bacterium is demonstrated.

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Wolbachia

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