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A Cell Free Assay to Study Chromatin Decondensation at the End of Mitosis

DOI :

10.3791/53407-v

December 19th, 2015

December 19th, 2015

10,074 Views

1Friedrich Miescher Laboratory, Max Planck Society, 2Nencki Institute of Experimental Biology, Polish Academy of Sciences

The molecular mechanisms of the decondensation of highly compacted mitotic chromatin are ill-defined. We present a cell-free assay based on mitotic chromatin clusters isolated from HeLa cells and Xenopus laevis egg extract that faithfully reconstitutes the decondensation process in vitro.

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Cell free Assay

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