1:18
Harvesting Small Bowel from Donor Mouse
1:20
1. performing Tail Pinch to Ensure Mouse is Anesthetized
2:46
2. clearing away tissue to allow ligation of small bowel
4:21
3. cleaning out small bowel contents before ligating ileum
5:20
5. exposing and ligating mesenteric vessels
6:18
6. exposing mesenteric artery which will be cut when small bowel is
6:42
7. exposing portal vein
7:28
8. ligating branch of portal vein
7:58
9. exposing other side of mesenteric artery
8:09
10. locating inferior vena cava
8:15
11. injecting heparin into the inferior vena cava
8:36
12. cutting mesenteric artery
8:54
13.clearing away tissue from mesentaric artery
9:23
14. cutting jejunal end of ligated small bowel
9:37
15. cutting away small bowel mesenteric tissue
10:27
16. cutting away mesenteric tissue until the portal vein is reached
10:56
17. cutting ileal end of ligated small bowel
11:19
18. cutting away mesenteric tissue
11:34
19. cutting portal vein
11:57
20. harvesting donor small bowel
12:09
Preparing Recipient Mouse for Small Bowel Transplantation
13:20
1. exposing inferior vena cava and aorta
13:29
2. clearing away tissue in order to apply ligatures
14:35
3. applying ligatures to inferior vena cava and inferior aorta
15:03
Connecting Donor Small Bowel Circulation to Recipient Circulation
15:15
1. placing donor small bowel in recipient mouse
15:56
puncturing inferior vena cava for veinotomy
16:17
3. performing veinotomy on inferior vena cava
16:31
4. repeat procedure for aorta
17:00
5. washing away blood from vessels with saline
17:15
6. suturing donor mesenteric artery to recipient aorta
19:59
7. suturing donor portal vein to recipient inferior vena cava
22:39
8. final suture for anastamosis of donor portal vein to recipient i
23:01
9. flipping donor small bowel in order to finish suture of mesenter
23:35
10. suturing donor mesenteric artery to other side of aorta
24:53
11. final suture for anastamosis of donor mesenteric artery to reci
25:56
12. releasing ligatures to open circulation to donor small bowel
26:47
Connecting Donor Small Bowel to Recipient Small Bowel
27:20
1. clamping ileal end of donor small bowel
27:39
2. cutting recipient jejunum
27:50
3. cutting ileal end of donor small bowel
28:04
4. suturing one side of donor ileum to recipient jejunum
29:10
5. flipping small bowel clamps in order to suture other side of ana
31:14
7. creating stoma for jejunal end of donor small bowel
32:02
8. suturing jejunal end of donor small bowel to stoma
32:40
9. cutting ligature to jejunal end of donor small bowel
33:14
Sewing up Abdominal Cavity
