Small Bowel Transplantation In Mice

0:16

Introduction

1:18

Harvesting Small Bowel from Donor Mouse

1:20

1. performing Tail Pinch to Ensure Mouse is Anesthetized

2:46

2. clearing away tissue to allow ligation of small bowel

4:21

3. cleaning out small bowel contents before ligating ileum

4:50

4. ligating ileum

5:20

5. exposing and ligating mesenteric vessels

6:18

6. exposing mesenteric artery which will be cut when small bowel is

6:42

7. exposing portal vein

7:28

8. ligating branch of portal vein

7:58

9. exposing other side of mesenteric artery

8:09

10. locating inferior vena cava

8:15

11. injecting heparin into the inferior vena cava

8:36

12. cutting mesenteric artery

8:54

13.clearing away tissue from mesentaric artery

9:23

14. cutting jejunal end of ligated small bowel

9:37

15. cutting away small bowel mesenteric tissue

10:27

16. cutting away mesenteric tissue until the portal vein is reached

10:56

17. cutting ileal end of ligated small bowel

11:19

18. cutting away mesenteric tissue

11:34

19. cutting portal vein

11:57

20. harvesting donor small bowel

12:09

Preparing Recipient Mouse for Small Bowel Transplantation

13:20

1. exposing inferior vena cava and aorta

13:29

2. clearing away tissue in order to apply ligatures

14:35

3. applying ligatures to inferior vena cava and inferior aorta

15:03

Connecting Donor Small Bowel Circulation to Recipient Circulation

15:15

1. placing donor small bowel in recipient mouse

15:56

puncturing inferior vena cava for veinotomy

16:17

3. performing veinotomy on inferior vena cava

16:31

4. repeat procedure for aorta

17:00

5. washing away blood from vessels with saline

17:15

6. suturing donor mesenteric artery to recipient aorta

19:59

7. suturing donor portal vein to recipient inferior vena cava

22:39

8. final suture for anastamosis of donor portal vein to recipient i

23:01

9. flipping donor small bowel in order to finish suture of mesenter

23:35

10. suturing donor mesenteric artery to other side of aorta

24:53

11. final suture for anastamosis of donor mesenteric artery to reci

25:56

12. releasing ligatures to open circulation to donor small bowel

26:47

Connecting Donor Small Bowel to Recipient Small Bowel

27:20

1. clamping ileal end of donor small bowel

27:39

2. cutting recipient jejunum

27:50

3. cutting ileal end of donor small bowel

28:04

4. suturing one side of donor ileum to recipient jejunum

29:10

5. flipping small bowel clamps in order to suture other side of ana

30:20

6. removing clamps

31:14

7. creating stoma for jejunal end of donor small bowel

32:02

8. suturing jejunal end of donor small bowel to stoma

32:40

9. cutting ligature to jejunal end of donor small bowel

33:14

Sewing up Abdominal Cavity

35:32

Conclusion