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Photobleaching Assays (FRAP & FLIP) to Measure Chromatin Protein Dynamics in Living Embryonic Stem Cells

DOI :

10.3791/2696-v

June 29th, 2011

June 29th, 2011

26,721 Views

1Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem

We describe photobleaching methods including Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Loss In Photobleaching (FLIP) to monitor chromatin protein dynamics in embryonic stem (ES) cells. Chromatin protein dynamics, which is considered to be one of the means to study chromatin plasticity, is enhanced in pluripotent cells.

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Photobleaching Assays

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