JoVE Logo
Faculty Resource Center

Sign In

Use of LysoTracker to Detect Programmed Cell Death in Embryos and Differentiating Embryonic Stem Cells (Video) | JoVE

DOI :

10.3791/4254-v

October 11th, 2012

October 11th, 2012

22,691 Views

1Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Keck School of Medicine, University of Southern California

We present a simple protocol to visualize regions of programmed cell death (PCD) in mouse embryos and differentiating embryonic stem (ES) cell cultures using a highly soluble dye called LysoTracker.

-- Views

Related Videos

article

Passaging HuES Human Embryonic Stem Cell-lines with Trypsin. (Video) | JoVE

article

Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction (Video) | JoVE

article

Induction and Analysis of Epithelial to Mesenchymal Transition (Video) | JoVE

article

Analysis of Apoptosis in Zebrafish Embryos by Whole-mount Immunofluorescence to Detect Activated Caspase 3 (Video) | JoVE

article

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus (Video) | JoVE

article

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR (Video) | JoVE

article

Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells (Video) | JoVE

article

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation (Video) | JoVE

article

Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury (Video) | JoVE

article

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry (Video) | JoVE

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved