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A Gradient-generating Microfluidic Device for Cell Biology

15.2K Views

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11:05 min

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August 30th, 2007

DOI :

10.3791/271-v

August 30th, 2007

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1Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology; Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital

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Gradient generating Microfluidic Device
Cell Biology
Cellular Behavior
Microfluidic Platform
Fluid Flows
High throughput Experiments
Soluble Concentration Gradients
PDMS based Microfluidic Devices
Growth Factors
Fluorescein Isothyiocyanate FITC dextran
Epidermal Growth Factor EGF
Fibroblasts
Cell Migration
Cell Motility
Cell Tracking Analysis

Chapters in this video

0:05

Introduction

0:21

Microfabrication of microfluidic device

0:56

Preparation of PDMS mixture

1:49

Patterning PDMS molds

2:20

Removing bubbles by vacuum

3:19

Assembly of microfluidic device

3:20

Cutting layers of microfluidic device

3:33

Creating channel inlet and outlet

3:59

Bonding the microfluidic device layers

6:55

Experiment: effect of EGF gradient on cell migration

7:13

Application of reagents

9:49

Experiment: cell migration

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