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The Mouse Cremaster Muscle Preparation for Intravital Imaging of the Microcirculation

DOI :

10.3791/2874-v

7:19 min

June 10th, 2011

June 10th, 2011

31,569 Views

1Department of Medical Pharmacology and Physiology, University of Missouri, 2Dalton Cardiovascular Research Center, University of Missouri

A tissue preparation is described for visualization and experimental manipulation of the living microcirculation. In anesthetized male mice, the thin, highly vascularized cremaster muscle is prepared for intravital microscopy to study microvascular networks including arterioles, capillaries and venules. This preparation is readily adapted for rats and hamsters.

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