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Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus

DOI :

10.3791/3140-v

October 7th, 2011

October 7th, 2011

10,743 Views

1Center for Autophagy Research, Department of Internal Medicine, UT Southwestern Medical Center, 2Department of Microbiology, UT Southwestern Medical Center

We describe a protocol to identify key roles of host signaling molecules in lytic replication of a model herpesvirus, gamma herpesvirus 68 (γHV68). Utilizing genetically modified mouse strains and embryonic fibroblasts for γHV68 lytic replication, the protocol permits both phenotypic characterization and molecular interrogation of virus-host interactions in viral lytic replication.

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Host virus Interaction

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