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Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids

DOI :

10.3791/53672-v

9:57 min

March 17th, 2016

March 17th, 2016

26,942 Views

1LABÉO Frank Duncombe, 2Unité de Risques Microbiens (U2RM, EA 4655), Normandy University, 3Hippolia Foundation

Here, we present a protocol for the development and validation of a quantitative PCR method used for the detection and quantification of EHV-2 DNA in equine respiratory fluids. The EHV-2 qRT-PCR validation protocol involves a three-part procedure: development, characterization of qRT-PCR assay alone, and characterization of the whole analytical method.

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QPCR Method

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